东北6种蒲公英瘦果形态聚类分析及分子系统学证据  

Achene morphology cluster analysis of Taraxacum F.H.Wigg. from Northeast China and molecule systematics evidence determined by SRAP

作　　者：李海娟[1] 赵鑫[1] 贾庆飞[1] 李天来[1] 宁伟[1] 

机构地区：[1]沈阳农业大学园艺学院、东北野菜种质异位保存圃与鉴定中心,设施园艺省部共建教育部重点实验室,辽宁沈阳110161

出　　处：《药学学报》2012年第8期1063-1069,共7页Acta Pharmaceutica Sinica

基　　金：国家农业部野生植物资源保护资助项目:东北野菜种质异位保存圃与鉴定中心建设(农计发[2010]29号)

摘　　要：采用瘦果形态与微形态学及分子系统学方法对东北地区6种蒲公英进行聚类分析与鉴定研究,为蒲公英属(Taraxacum F.H.Wigg.)分类及亲缘关系研究提供瘦果形态(微形态)学及分子系统学证据。利用体式数码光学解剖镜和电子探针显微镜,对东北地区6种蒲公英瘦果进行观察比较,根据瘦果大小、形状、颜色、喙基比例及微形态表面纹饰特征、刺瘤分布与大小等18个性状指标进行聚类分析。同时利用SRAP分子标记对6种蒲公英(Taraxacum)亲缘关系进行分子系统学分析论证。结果表明,蒲公英属种间瘦果形态特征差异明显,尤其是刺瘤分布及大小、瘦果颜色与大小等特征;基于瘦果形态(微形态)特征及聚类结果将丹东蒲公英(T.antungense Kitag.)和卷苞蒲公英(T.urbanum Kitag.)归并为一个种。瘦果形态学聚类分析和SRAP分子标记的聚类结果与《中国植物志》检索表中的类群划分结果一致。蒲公英属植物瘦果形态特征稳定保守,可根据瘦果形态特征组合差异性进行种间划分和亲缘关系分析;基于瘦果形态学及SRAP分子标记的两种聚类结果均支持《中国植物志》对这6种蒲公英的分类结果。The achenes morphological and micro-morphological characteristics of six species of genus Taraxacum from Northeastern China as well as SRAP cluster analysis were observed for their classification evidences.The achenes were observed by microscope and EPMA.Cluster analysis was given on the basis of the size,shape,cone proportion,color and surface sculpture of achenes.The Taraxacum inter-species achene shape characteristic difference is obvious,particularly spinulose distribution and size,achene color and achene size;with the Taraxacum plant achene shape the cluster method T.antungense Kitag.and the T.urbanum Kitag.should combine for the identical kind;the achene morphology cluster analysis and the SRAP tagged molecule systematics’s cluster result retrieves in the table with ＂The Chinese Flora＂.The class group to divide the result is consistent.Taraxacum plant achene shape characteristic stable conservative,may carry on the inter-species division and the sibship analysis according to the achene shape characteristic combination difference;the achene morphology cluster analysis as well as the SRAP tagged molecule systematics confirmation support dandelion classification result of ＂The Chinese Flora＂.

关 键 词：蒲公英 瘦果形态 SRAP 聚类分析 

分 类 号：R931[医药卫生—生药学]