冷冻对轮虫休眠卵萌发率的影响  

Influence of freezing on hatching rates of resting eggs from several species of rotifers

作　　者：刘青[1] 金送笛[1] 刚健[1] 覃永秋 陈红艳 李永函[1] 

机构地区：[1]农业部海洋水产增养殖重点开放实验室,大连水产学院大连116023

出　　处：《中国水产科学》1999年第4期39-42,共4页Journal of Fishery Sciences of China

基　　金：农业部九五重点项目!( 渔95 - B- 96 - 07 - 05)

摘　　要：对在不同冷冻时间和冷冻强度下臂尾轮虫休眠卵的萌发率进行了测试，结果显示，褶皱臂尾轮虫（ Ｂｒａｃｈｉｏｎｕｓ ｐｌｉｃａｔｉｌｉｓ） 休眠卵在－ １８ ℃冷冻２０ ｄ ，萌发率提高１３ ．９ ％ ；－ ３０ ℃冷冻６０ ｄ ，萌发率提高１４ ．４ ％ ；室外经整个冬季冻后，萼花臂尾轮虫（ Ｂ．ｃａｌｙｃｉｆｌｏｒｕｓ） 、壶状臂尾轮虫（ Ｂ．ｕｒｃｅｕｓ） 休眠卵的萌发率平均提高了１ ～３ 倍，而角突臂尾轮虫（ Ｂ．ａｎｇｕｌａｒｉｓ） 的萌发率却降低了近１ 倍。－ ８０ ℃强冷冻２ ｄ，褶皱臂尾轮虫休眠卵萌发率从３９ ．５ ％ 降至１ ．６ ％ 。同时，本文探讨了冷冻影响轮虫休眠卵萌发的机理以及冷冻处理在北方轮虫土池生产中的实际意义。Hatching rates of resting eggs from several species of rotifers were determined in various freezing periods and at different temperatures.Hatching rates of resting eggs from Brachinous plicatilis increased by 13.9% at-18℃ in a freezing period of 20 d and by 14.4%, at-30℃ in a freezing period of 60 d. Hatching rates of resting eggs from B.calyciflorus and B.urceus, exposed to dry and cold outdoor throughout a winter in Northeast China,rose from 100% to 300%,while approximately 50% of the resting eggs of Bangularis developed at the same conditions.The hatching rates of resting eggs from B.plicatilis decreased from 39.5% to 1.6% at -80℃ in a freezing period of 2 d.The developing mechanism of the frozen resting eggs and a practical imporance of freezing treatments in rotifer culture in earthen ponds in Northern China were discussed.

关 键 词：轮虫 休眠卵 冷冻 萌发率 

分 类 号：Q959.181[生物学—动物学]