白藜芦醇合酶基因对草莓遗传转化的研究  

Transformation of Resveratrol Synthase Gene in Fragaria ananassa

作　　者：林碧英[1] 钱昆[2] 连肖华[3] 林忠平[4] 张瑜 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]山东杏林科技职业学院,山东济南250200 [3]福建农林大学作物学院,福建福州350002 [4]北京大学生命科学院,北京100871 [5]福建省农业区划研究所,福建福州350003

出　　处：《热带作物学报》2012年第5期848-853,共6页Chinese Journal of Tropical Crops

基　　金：福建省科技厅重点项目(No.2009N1003)

摘　　要：根据已公布的白藜芦醇合酶基因(RS)序列,利用RT-PCR方法从川鄂爬山虎总RNA中获得完整的RScDNA序列;根据已公布的草莓果实特异性启动子RJ39序列,利用PCR方法从草莓基因组DNA中获得该启动子序列;构建特异性植物表达载体pCAMBIA3300-RJ39-RS-Tnos;在根癌农杆菌介导下,利用叶盘法转化草莓,通过PPT筛选和PCR检测,获得18个转基因株系,对其中7个株系进行Southern印迹杂交鉴定,均杂交出条带。According to the published nueleotide sequences of RS（Resveratrol synthase）and the fruit specific promoter R J39 in NCBI, we cloned the whole RS cDNA gene from the parthenocissus henryana, and isolated the R J39 promoter from strawberry by RT-PCR and PCR respectively. The plant specific expression vector pCAMBIA3300-RJ39-RS-Tnos was constructed, and transformed into the strawberry through Agrobacterium tumefaciens-mediated. The result indicated that 18 transgenie plants were obtained by PPT screening and PCR detection, 7 of these plants were detected by Southern blottin~ randomly, all the events showed nositive results.

关 键 词：白藜芦醇合酶基因 草莓 遗传转化 

分 类 号：Q785[生物学—分子生物学]