四磨汤对糖尿病大鼠降钙素基因相关肽及饥饿素的影响  

Effects of Simo decoction on calcitonin gene-related peptide and ghrelin in diabetic rats

作　　者：戴迟兵[1] 刘娜[1] 钱伟[1] 侯晓华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化内科,武汉430022

出　　处：《中华消化杂志》2012年第7期441-444,共4页Chinese Journal of Digestion

基　　金：国家973计划项目（2009CB523002）

摘　　要：目的研究四磨汤对糖尿病大鼠降钙素基因相关肽（CGRP）及饥饿素的影响。方法48只SD大鼠分为健康对照组、糖尿病组、糖尿病四磨汤低剂量治疗组、糖尿病四磨汤中剂量治疗组、糖尿病四磨汤高剂量治疗组及糖尿病中剂量预防组，每组8只。予糖尿病组及各干预组大鼠1％链脲佐菌素腹腔注射造模，健康对照组仅予柠檬酸母液注射。造模成功后8周，各干预组大鼠予低（0．15ml·kg^-1·d^-1）、中（1．5ml·kg^-1·d^-1）、高（3．0ml·kg^-1·d^-1）剂量四磨汤灌胃2周，对照组及糖尿病组子等体积0．9％NaCl溶液灌胃。预防组于造模成功后即开始予1．5ml·kg。·d。四磨汤灌胃10周。药物干预结束后处死大鼠，取胃窦组织行实时PCR检测饥饿素、CGRP的表达。数据处理采用单因素方差分析和z检验。结果糖尿病大鼠胃窦饥饿素基因水平较对照组无明显变化，但低、中剂量四磨汤干预后饥饿素表达水平高于糖尿病组（1．45±0．34、1．87±0．68比0．87±0．28． dunnett’s T3检验，P〈0．05），高剂量四磨汤干预对糖尿病大鼠饥饿素的表达无影响，四磨汤中剂量预防或治疗对糖尿病大鼠饥饿素的影响差异无统计学意义；糖尿病大鼠胃窦CGRP基因水平明显高于对照组（4．61±1．67比1．06±0．40，t=5．843，P〈0．01），低剂量四磨汤干预对糖尿病大鼠CGRP表达无影响，四磨汤中、高剂量干预可下调糖尿病大鼠CGRP的表达，四磨汤中剂量预防或治疗对糖尿病大鼠CGRP表达的影响差异无统计学意义。结论四磨汤可促进糖尿病大鼠胃窦饥饿素的基因表达。糖尿病大鼠胃窦CGRP基因表达显著增加，但四磨汤可下调糖尿病大鼠胃窦CGRP的基因表达水平。[Abstract] Objective To investigate the effects of Simo decoction on ghrelin and calcitonin generelated peptide （CGRP） in diabetic rats. Methods A total of 48 SD rats were divided into healthy conlrol group, diabetes group （DM）, DM treated with low dose Simo decoction group （DM TL）, DM treated with medium dose Simo decoction group （DM-TM）, DM treated with high dose Simo decoction group （DM-TH） and DM prevented with medium dose Simo decoction group （DM- PM） ; there were eight rats in each group. The rats of DM group and other intervention groups were intraperitoneally injected with 1 % streptozotocin （STZ） to establish the model. The rats of healthy control group were injected with solvent. At eight weeks after the model successfully established, the rats of each intervention group were gavaged with low dose of Simo decoction （0.15 ml·kg^-1·d^-1） , medium dose of Simo decoction （1.5 ml·kg^-1·d^-1） and high dose of Simo decoction （3.0 ml ·kg^-1·d^-1 ） for two weeks ; the rats of control group and DM groups were gavaged with equal volume of 0.9% NaC1. The rats of DM-PM group were gavaged with Simo decoction at 1.5 ml·kg^-1·d^-1 for 10 weeks after the model successfully established. Rats were sacrificed at the end of drug intervention and gastric antrum tissues were dissociated for detecting the expression of ghrelin and CGRP by real time polymerase chain reaction （PCR）. The data were analyzed by one way ANOVA and t test. Results There was no significant difference in ghrelin expression at mRNA level in gastric antrum tissues between diabetic rats and control group. However after intervened by low dose and medium dose of Simo decoction, the ghrelin expression increased （1.45±0.34, 1.87 ±0.68 compared with 0.87 ±0.28, Dunnett＇s T3 test, P〈0.05）. There was no effects of high dose Simo decoction on the expression of ghrelin in diabetic rats. There was no significant difference in ghrelin between DM-TM and DM-PM. The expression of CGRP at mRNA level in gastri

关 键 词：四磨汤 糖尿病 幽门窦 胃促生长素 降钙素基因相关肽 大鼠 Sprague—Dawley 

分 类 号：R285.5[医药卫生—中药学]