褪黑素对AD模型小鼠脑内小胶质细胞的影响  

The effects of melatonin on the activity of microglia in the brains of AD mice

作　　者：崔卫刚[1,2] 李君艳[3] 王庆志[1] 彭裕文[2] 李瑞锡[2] 

机构地区：[1]新乡医学院人体解剖学教研室,河南新乡453003 [2]复旦大学上海医学院解剖组胚系,上海200032 [3]新乡医学院第三附属医院肿瘤科,河南新乡453003

出　　处：《中国临床解剖学杂志》2012年第4期417-420,425,共5页Chinese Journal of Clinical Anatomy

基　　金：河南省卫生厅重点课题(200802008);新乡医学院重点研究领域招标课题(ZD2011-28)

摘　　要：目的探讨褪黑素对APP/PS1转基因AD模型小鼠脑内小胶质细胞和炎症细胞因子COX-2的抑制效应。方法 AD转基因小鼠随机分为褪黑素处理组和对照组。14 d后,取海马通过免疫荧光化学染色检测小胶质细胞、老年斑位置情况;Western blot、ELISA方法分别检测CD11b、COX-2的变化;Western blot检测炎症信号通路TLR/NF-κB的变化。结果免疫荧光化学显示,老年斑周围有大量活化的小胶质细胞聚集;Western blot、ELISA结果显示褪黑素组小鼠脑内CD11b、COX-2表达显著减少;褪黑素组小鼠TLR/NF-κB炎症信号通路的TLR2、NF-κB-p65表达减少。结论褪黑素可能通过抑制TLR/NF-κB炎症信号通路来抑制小胶质细胞活化并抑制炎症细胞因子COX-2的分泌。Objectives To investigate the changes ofmicroglia activity, and the expression of COX-2 affected by melatonin in APP/PS1 transgenic mice. Methods The mice of AD model were divided randomly into 2 groups： MT-treated group and vehicle treated group. We tested the relations of microglia and AI3 plaque in AD model by double immunofluorescence staining, and observed the changes of CD1 lb and COX-2 by western blot and ELISA, respectively. We also tested the expression of TLR/NF-κB inflammatory signaling pathway. Results CD1 lb-stained microglias were observed predominantly at the sites of Aβ deposition. MT reduced the expression of CD1 lb and COX-2. The protein levels of TLR2 and NF-κB-p65 were decreased in MT-treated group. Conclusions MT may inhibit the activation of mieroglia and the expression of COX-2 through a pathway associated with TLR/NF-κB inflammatory signaling pathway.

关 键 词：褪黑素 小胶质细胞 痴呆 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]