全反式维甲酸促鼠胚神经干细胞向神经元分化中BMP-2的表达及意义  

Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro

作　　者：钟德君[1] 李森[1] 康敏[2] 徐双[1] 魏书一[1] 王清[1] 王松[1] 

机构地区：[1]泸州医学院附属医院脊柱外科,四川泸州646000 [2]泸州医学院附属医院内科,四川泸州646000

出　　处：《中国临床解剖学杂志》2012年第4期426-430,共5页Chinese Journal of Clinical Anatomy

基　　金：泸州市重点科技计划项目(2008-23-5);四川省卫生厅科研项目(2008-527-080178)

摘　　要：目的探讨全反式维甲酸(all-trans-retinoic-acid,ATRA)体外促NSCs分化为神经元的过程中,BMP-2表达的变化及意义。方法 1.0μmol/L ATRA诱导体外培养的鼠胚神经干细胞,诱导3、5、7、9 d后,采用免疫细胞化学、qPCR、Western-Blot检测BMP-2表达变化。结果无ATRA干预时BMP-2表达量较高,随着诱导时间的延长,BMP-2表达逐渐降低,分化7～9 d时表达最低。结论 BMP-2在NSCs体外分化的的早期表达较高,在NSCs分化为神经元过程中逐渐下调,表明ATRA诱导NSCs向神经元的分化与BMP-2信号的抑制有关。Objective To explore the expression and effect of BMP-2 during the rat embryonic stem cells（NSCs） induced by all-trans-retinoic acid（ATRA）differentiating into neurons in vitro. Methods The rat embryonic neural stem cells were differentially induced with 1.0 μmol/L ATRA in vitro. The expression of BMP-2 was respectively investigated by immunocytochemistry, real-time PCR and western blot at the time of inducing 3d, 5d, 7d and 9d, and compared with that of untreated cells in control group. Results The highest expression of BMP-2 was at the time of inducing 3d, and then it decreased from inducing 5d following the NSCs differentiating. There was no significant difference between 7d and 9d groups. Conclusions The expression of BMP-2 decreases in the progress of NSCS differentiating into neurons in vitro, which indicate that, the inhibition of BMP2 signal pathway participate the differentiation process of NSCs.

关 键 词：神经干细胞 全反式维甲酸 BMP-2 分化 表达 

分 类 号：R338.1[医药卫生—人体生理学]