RP-HPLC测定止痛膏中大黄酸、大黄素和大黄酚的含量  

Content Determination of Rhein,Emodin and Chrysophanol in Zhitonggao by RP-HPLC

作　　者：闵云山[1] 焦正花[1] 张小华[1] 杨晔[1] 

机构地区：[1]甘肃省中医院,甘肃兰州730050

出　　处：《中国中医药信息杂志》2012年第7期52-53,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省自然科学基金(1010RJZ206)

摘　　要：目的建立测定止痛膏中大黄酸、大黄素和大黄酚含量的方法。方法采用反相高效液相色谱法,十八烷基键合硅胶柱分离大黄酸、大黄素和大黄酚,以甲醇-水(85∶15)为流动相,流速为1.0 mL/min,检测波长254 nm。结果大黄酸在0.208～1.040μg范围内线性关系良好(r=0.9990,n=5),平均加样回收率为98.37%(RSD=0.57%,n=5);大黄素在0.192～0.96μg范围内线性关系良好(r=0.9997,n=5),平均加样回收率为98.45%(RSD=0.99%,n=5);大黄酚在0.596～2.980μg范围内线性关系良好(r=0.999 5,n=5),平均加样回收率为98.18%(RSD=1.50%,n=5)。结论本方法简单、灵敏、准确、重复性好,可用于该制剂的质量控制。Objective To establish the method to determine the contents of rhein,emodin and chrysophanol in Zhitonggao.Methods RP-HPLC method was adopted with Hypersil ODS C18 as column.The mobile phase consisted of methanol-water（85∶15） with a flow rate of 1.0 mL/min.The detection wavelength was 254 nm.Results The linear correlation of rhein was good in the range of 0.208-1.040 μg（r=0.999 0,n=5） and the recovery was 98.37%（RSD=0.57%,n=5）.The linear correlation of emodin was good in the range of 0.192-0.96 μg（r=0.999 7,n=5） and the recovery was 98.45%（RSD=0.99%,n=5）.The linear correlation of chrysophanol was good in the range of 0.596-2.980 μg（r=0.999 5,n=5） and the recovery was 98.18%（RSD=1.50%,n=5）.Conclusion The method is simple,sensitive and accurate,with good reproducibility.It can be applied to control the quality of the preparation.

关 键 词：止痛膏 大黄酸 大黄素 大黄酚 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]