血管紧张素-(1-7)对醛固酮激活大鼠肾间质成纤维细胞的影响  

Influence of angiotensin-(1-7) on cell activation in rat renal interstitial fibroblasts induced by aldosterone

作　　者：谭剑[1] 夏纪毅[2] 贺红焰[3] 陈枫[2] 樊均明[1] 欧三桃[1] 刘建[1] 

机构地区：[1]泸州医学院附属医院肾病内科,四川泸州646000 [2]泸州医学院附属医院分子与免疫研究室,四川泸州646000 [3]泸州医学院附属医院病理教研室,四川泸州646000

出　　处：《细胞与分子免疫学杂志》2012年第8期808-810,814,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：四川省卫生厅项目(110365)

摘　　要：目的:观察血管紧张素-(1-7)[Ang-(1-7)]对醛固酮(ALD)激活大鼠肾间质成纤维细胞(NRK-49F)及细胞外基质分泌的影响并初步探讨其机制。方法:体外培养大鼠肾间质成纤维细胞,分为对照组、Ang-(1-7)组、ALD组、ALD+Ang-(1-7)组。培养48 h后,运用免疫细胞化学染色法检测细胞激活标志物α-平滑肌肌动蛋白(α-SMA)的表达;酶联免疫吸附法(ELISA)检测上清液中Ⅰ型胶原(ColⅠ)的含量;按上述实验分组干预30 min后,运用Western blot法检测细胞裂解液中磷酸化ERK1/2(pERK1/2)及总ERK1/2(tERK1/2)的表达。结果:与对照组比较,ALD组及ALD+Ang-(1-7)组细胞α-SMA、ColⅠ及ERK1/2相对表达量则显著增加(P<0.05);与ALD组比较,ALD+Ang-(1-7)组细胞α-SMA、ColI及ERK1/2相对表达量明显减少(P<0.05)。结论:Ang-(1-7)可抑制ALD介导的肾间质成纤维细胞激活,减少细胞外基质成份ColⅠ的分泌,ERK1/2信号通路在这一过程中具有一定作用。AIM： To explore the influence of angiotensin-（1-7） [Ang-（1-7）] on cell activation and extracellular matrix secretion in rat renal interstitial fibroblasts（NRK-49F） induced by aldosterone（ALD）.METHODS： The NRK-49F cells were cultured in vitro,and then were divided into control group,ALD group,Ang-（1-7） group,and ALD＋Ang-（1-7） group.When the cells were cultured for 48 h,the expression of α-smooth muscle actin（α-SMA）（a sign of cell activation） was detected by immunocytochemistry;the level of collagen type Ⅰ（ColⅠ） in the cultured supernatant was measured by enzyme-linked immunosorbent assay（ELISA）.When the cells were cultured for 30 min,the expressions of phosphorylated and total ERK1/2（pERK1/2,tERK1/2） in the cell lysate were detected by Western blotting.RESULTS： Compared with control group,the expressions of α-SMA,ColⅠ and the Phos/Total ERK1/2 ratio in ALD group and ALD＋Ang-（1-7） group increased significantly（P0.05）.Compared with ALD group,the expressions of α-SMA,ColⅠ and the Phos/Total ERK1/2 ratio in ALD＋Ang-（1-7） group decreased significantly（P0.05）.CONCLUSION： Ang-（1-7） can inhibit ALD-induced cell activation and decrease the secretion of ColⅠ in rat renal interstitial fibroblasts.Inhibition of ERK1/2 pathway may play an important role in this process.

关 键 词：血管紧张素-(1-7) 醛固酮 肾间质 成纤维细胞 细胞外信号调节激酶 

分 类 号：R392.12[医药卫生—免疫学]