油茶芽苗砧嫁接口不同发育时期差异蛋白质分析  

Analysis of differential proteins in nurse seed grafted unions of Camellia oleifera at its different developmental stages

作　　者：冯金玲[1] 杨志坚[1] 陈辉[1] 

机构地区：[1]福建农林大学,福州350002

出　　处：《应用生态学报》2012年第8期2055-2061,共7页Chinese Journal of Applied Ecology

基　　金：科技人员服务企业行动项目(2009GJC40006);福建省科技平台建设项目(2010N2001);福建省农业科技重大项目(2010N5006)资助

摘　　要：以油茶芽苗砧嫁接口为试验材料,运用蛋白质双向电泳结合质谱技术研究嫁接口不同发育时期(嫁接后第4、8、16、22、29和35天)蛋白质组的变化.结果表明:分析获得40个差异蛋白,其中9个差异蛋白可能与芽苗砧嫁接口愈合有关.嫁接一方面刺激嫁接口基因转录和蛋白质翻译、信号传导、能量代谢、物质合成和激素调节;另一方面刺激部分酶活性,提高细胞抗性,促进油茶芽苗砧嫁接口愈合.By using two-dimensional electrophoresis and mass spectrometry,this paper studied the changes of the proteome in nurse seed grafted unions of Camellia oleifera at its different developmental stages（4,8,16,22,29,and 35 days after grafting）.A total of 40 differential proteins were detected,9 of which could be related to the healing of nurse seed grafted unions.Grafting stimulated the grafted union＇s gene transcription and protein translation,signal transduction,energy metabolism,and compound synthesis and hormone regulation,and activated some enzymes activities,enhanced cell resistance,and promoted the union＇s healing.

关 键 词：油茶 芽苗砧嫁接 蛋白质组学 愈合 

分 类 号：S794.4[农业科学—林木遗传育种]