乙型肝炎病毒S区基因变异的检测及其意义  

Detection and significance of gene variation in hepatitis B virus S region

作　　者：荆永正 浦声波 贾玉平[3] 杨建 胡克义 乔明刚 于学英 张杰 

机构地区：[1]山东省蓬莱市人民医院检验科,265600 [2]蓬莱市中医院检验科 [3]山东省潍坊市人民医院检验科 [4]山东省烟台市中心血站

出　　处：《中华医学杂志》2012年第27期1882-1885,共4页National Medical Journal of China

基　　金：山东省科技攻关资助项目（2004GG22008030）

摘　　要：目的探讨乙型肝炎病毒（HBV）S基因变异规律，分析HBV基因变异对病毒生物学行为的影响。方法收集2010年1月1日至2011年6月20日山东省蓬莱市人民医院乙肝患者血清或血浆标本95例，采用PCR结合DNA测序对HBVS区基因进行多态性检测，并应用DNAstar软件进行比对和分析。结果①有15例HBV标本S基因测序成功。3例标本在HBsAg“a”决定簇（AA124～147位）AA126发生I→T突变。②19例HBV标本共出现67处碱基突变，PreS1基因14处，PreS2基因6处。同义突变nt3036T→C、nt3039TIG和nt3066C→T以及错义突变L88V出现于所有标本，均位于PreS1基因。结论S区基因某些位点的突变见于所有扩增成功标本，提示突变热点可能有地域性，探讨位点突变的意义，有助于阐明HBV致病的分子机制，对乙肝的基因诊断及预防提供新的实验依据。Objective To explore the variations of gene S in hepatitis B viruses of hepatitis B patients and provide experimental evidences for the mutation analysis of viral gene. Methods The virus DNA load in hepatitis B patient donors was detected by real-time polymerase chain reaction （PCR） and gene sequence analysis. And a comparison was made with standard strain by the software DNAstar. Results （1） Gene S was successfully amplified and sequenced in 15 hepatitis B patients. Three samples had I→T mutation at residue 126 in HBsAg ＂ a＂ antigenic determinant. （2） Sixteen hepatitis B patients had 67 nucleotide mutations, including 14 residues in PreS1 and 6 residues in PreS2. Mutations nt 3036 T→C, nt 3039 T→G, nt 3066 C→T and L88V existed in PreS1 gene in all samples. Conclusion HBV genome is susceptible to nucleotide mutations. Some residues have geographically restricted mutations in gene S region. And understanding the significance of these mutations may help clarify the pathogenesis of hepatitis B and provide new experimental evidence for its gene diagnosis and prevention.

关 键 词：肝炎病毒 乙型 多态性 单核苷酸 遗传变异 

分 类 号：R4[医药卫生—临床医学] R51