西洋参皂苷抑制缺氧性神经细胞凋亡机制的研究  

Study of mechanism of Panax quinquefolium L. saponin suppressing neurons apoptosis induced by anoxia

作　　者：胡国柱[1] 漆琼瑶[2] 高幼奇[1] 文珠[3] 

机构地区：[1]江西省人民医院神经内科,南昌330006 [2]南昌大学医学院 [3]江西省医学科学研究所,南昌330006

出　　处：《中药药理与临床》2012年第2期57-61,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省科技厅社会发展重点科研项目"补气中药抗神经细胞缺氧性凋亡作用研究";项目编号:2007BS22602

摘　　要：目的:探讨西洋参皂苷(PQLS)抑制缺氧诱导的胚鼠大脑皮层神经细胞凋亡的作用机理。方法:正常对照组、凋亡阳性组、西洋参皂苷0.025g/L组、0.05g/L组、0.1g/L组、0.2g/L组;SD胚大鼠原代大脑皮层神经细胞体外无血清缺氧-复氧培养;应用MTT、DNA电泳、免疫细胞化学染色等方法观察西洋参皂苷的适宜剂量、DNA凋亡条带及凋亡调节蛋白Bcl-2、Bax、Caspase-3的表达率。结果:①缺氧条件下PQLS在0.05～0.1g/L促进缺氧的神经细胞增殖较凋亡阳性组提高31.17%～37.65%(P﹤0.01)。②0.05～0.2g/L的PQLS使缺氧的神经细胞DNA只出现3条凋亡条带(凋亡阳性组6条带),且条带浓度也明显降低。③0.025g/L～0.1g/L PQLS显著减少缺氧的神经细胞表达Bax(67.02±1.69%～57.97±2.6%,凋亡阳性组72.19±3.97%,P<0.05～0.01)和Caspase-3(62.27±2.02%～50.34±2.65%,凋亡阳性组68.62±1.40%,P<0.05～0.01);提高缺氧的神经细胞表达Bcl-2(44.22±3.73%～63.76±2.96%,凋亡阳性组35.61±2.16%,P<0.01)和Bcl-2/Bax比值(0.69±0.11～1.11±0.04,凋亡阳性组0.56±0.11,P<0.05～0.01)。结论:西洋参皂苷通过降低缺氧的神经细胞表达Bax、Caspase-3,提高Bcl-2的表达及Bcl-2/Bax比例阻断缺氧诱导的神经细胞凋亡,保护缺氧的神经细胞。To explore the mechanisms of Panax quinquefolium L. saponin （PQLS） suppressing apoptosis of embryonic rat cerebral corti- cal neurons induced by anoxia. Methods： The Experiments were divided into normal control, apoptosis positive group, PQLS 0. 025g/L group, PQLS 0.05g/L group, PQLS 0. lg/L group, PQLS 0.2g/L group. Primary anoxia-reoxygenation culture of SD embryonic rat cerebral cortical neurons was performed in serum-free in vitro. The appropriate dosage of PQLS, apoptosis strip of fragmented DNA, and expression rates of anti-apoptotic protein Bcl-2 and apoptosis proteins Bax and Caspase-3 were determined by MTI＇ assay, DNA electrophores;s and im- munocytochemistry. Resulls：（!）With increasing concentration in PQLS 0.05g/L -0. lg/L the proliferation rates of anoxic neurons still in- creased gradually comparing with apoptosis positive group （31.17% -37.65%, P 〈 0.01 ）. （~）In PQLS 0.05g/L-0.2g/L there were three strips of fragmented DNA of anoxic neurons to be watched, which was less than in apoptosis positive group not only in numbers （ six strips） but also in vdume. （~With increasing concentration in PQLS 0.025g/L - 0. lg/L the anoxic neurons expressed Bax would decrease gradually （67.02±1.69% -57.97±2.6% ） comparing with it in apoptosis positive group （72.19±3.97%, P 〈 0.05-0.01 ）, the anoxic neurons expressed Caspase-3 would decrease gradually （62.27±2.02%-50.34±2.65% ） comparing with it in apoptosis positive group （68.62±1.40%, P 〈 0.05-0.01 ）, the anoxic neurons expressed Bcl-2 would increase gradually （44.22±3.73 %- 63.76±2.96% ） , comparing with it in apoptosis positive group （ 35.61±2.16%, P 〈 0.01 ）, and the Bcl-2/Bax ratio would increase gradually （0.69±0. 11 1.11-0. 04） comparing with it in apoptosis positive group （0.56±0.11, P 〈 0.05-0.01 ）. Conclusion： By means of reducing the anoxic neurons ex- pressed Bax and Caspase-3, and increasing the anoxic neurons expressed Bcl-2 and ratio of anoxic neurons expressed Bcl-2

关 键 词：西洋参皂苷 神经细胞 原代培养 缺氧-复氧 凋亡 DNA Bcl-2 BAX Caspase-3 

分 类 号：R285[医药卫生—中药学]