全蚕粉对2型糖尿病大鼠胰岛素受体信号传导通路的影响  

Effects of Silkworm Powder on signaling pathway in Type 2 diabetic rat

作　　者：曹育[1,2] 黄亦琦[1] 黄恺飞[1] 许光辉[1] 王梅兰[1] 

机构地区：[1]厦门市医药研究所,厦门361008 [2]山西中医学院,太原030024

出　　处：《中药药理与临床》2012年第2期126-129,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：福建省自然科学基金(No:2011D006)

摘　　要：目的:观察全蚕粉对2型糖尿病胰岛素受体信号传导通路的影响,探讨全蚕粉逆转2型糖尿病胰岛素抵抗的分子作用机理。方法:以高热量饲料喂养大鼠8周加小剂量链脲佐菌素(STZ,25mg/kg)腹腔注射建立2型糖尿病大鼠模型。应用全蚕粉进行干预,以阿卡波糖片作为阳性对照药,用Real-time PCR和Western blotting技术,观察全蚕粉对Akt,InsR,PI3K基因及其蛋白表达的影响。结果:与模型组比较,全蚕粉组0.5、1.0、2.0g/kg肝组织Akt,InsR,PI3K mRNA的相对表达量无显著性差异,肝组织Akt,InsR,PI3K蛋白表达量显著上调(P﹤0.05)。结论:全蚕粉对2型糖尿病大鼠的影响可能在翻译水平上通过PI3K信号传导途径增强胰岛素生物效应,从而逆转2型糖尿病大鼠胰岛素抵抗。To study the effects of Silkworm Powder（SP） on glucose and lipid metabolism in Type 2 Diabetic rats and exam the effects of Silkworm powder on PI3K/AKT signaling pathway in Type 2 Diabetic rats. In order to understand the mechanisms of SP＇protective effects on Type 2 Diabetesmellious. Methods： Type 2 Diabetic model was induced. Rats were randomly divided into five groups. To investigate the effects of SP on PI3K/AKT signaling pathway in hepatic tissue by real time-PCR and Western blotting after 6 weeks of intragastric administra- tion with vehicle. Results： Compared with type 2 DM groups, no significant changs in the mRNA level of Akt, InsR, PI3K in all treatment groups . Tile protein expl ession of Akt in heptic tissue significantly increased in middle and high dose of groups ;The protein expression of In- sR and PI3K in hepatic tissue also significantly increased（ P 〈 0.05）. Conclusion： The results of present study demonstrated that SP can ef- fectively improve the gly-lipid metabolim and have an improvement on IR. Silkworm powder can improve PI3K/AKT signaling pathway in Type 2 Diabetic rats .

关 键 词：全蚕粉 2型糖尿病 PI3K 信号传导通路 

分 类 号：R285[医药卫生—中药学]