补中益气汤对脾虚大鼠胃粘膜TFF1mRNA与蛋白表达的影响  

Effect of Buzhongyiqi Decoction on the expression of gastric mucosa TFF1 and protein in rats with the syndrome of Spleen Deficiency

作　　者：刘晓玲[1] 王汝俊[1] 莫旺福[1] 李锦[1] 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中药药理与临床》2012年第3期25-28,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:观察脾虚大鼠胃粘膜TFF1mRNA与蛋白表达变化及补中益气汤对其表达的影响,并探讨相关作用机制。方法:SD大鼠随机分为空白对照、脾虚损伤、补中益气汤、非脾虚损伤组。脾虚损伤组、补中益气汤组大鼠给予100%大黄水煎剂灌胃,一天2次,连续10天。第11天补中益气汤组给予补中益气汤灌胃治疗7天。第18天,除空白对照组外,另三组灌服消炎痛溶液造成胃粘膜损伤,采用逆转录PCR(RT-PCR)检测TFF1mRNA,采用双抗体两步夹心酶联免疫吸附法(ELISA)检测TFF1蛋白表达的变化。结果:与非脾虚损伤组比较,脾虚损伤组大鼠胃粘膜TFF1mRNA、TFF1蛋白表达明显降低;与脾虚损伤组比较,补中益气汤组大鼠胃粘膜TFF1mRNA、蛋白明显上调。结论:脾虚大鼠消炎痛攻击后胃粘膜TFF1mRNA、蛋白表达显著下调,提示脾虚大鼠胃粘膜防御能力减弱,补中益气汤可提高脾虚大鼠消炎痛攻击后胃粘膜TFF1mRNA与蛋白的表达,这可能是补中益气汤保护胃粘膜,降低脾虚大鼠胃粘膜易损性的机理之一。To observed the expression of gastric mucosa TFF1 mRNA and protein in the Spleen Deficient rats and the effect of Buzhongyiqi- tang, the related mechanisms were discussed. Methods： The rats were randomly divided into the spleen deficiency with injury group, The modelof spleen deficiency was copied by Dahuang, and Buzhongyiqitang group , the blank control group, and the non- spleen deficiency with injury group according to their weight. The spleen deficiency with injury group and the Buzhongyiqitang group were administrated by 100% DaHuang solution, twice a day ,for 10 days. Then the Buzhongyiqitang group was treated by Buzhongyiqitang for 7 days, twice a day. In addi- tion to the control group, the other three groups were fed with indomethacin solution to cause mucosa injruy after treatment. RT-PCR was usedto assay TFF1 mRNA, ELISA was used to detect the expressibn of TFF1 protein. Results： Compared with the non-spleen deficiency with inju- ry group, the expression of gastric mucosa TFF1 mRNA and protein in the spleen deficiency with injury group were decreased significantly（ P 〈 0.05 ,P 〈0.01 ） ; Compared with the spleen deficiency with injury group, the expression of gastric mucosa TFF1 mRNA and protein in the Buzhongyiqitang group were increased significantly（ P 〈 0.05 ）. Conclusion： The expression of gastric mucosa TFF1 mRNA and protein in the spleen deficiency with injury group were decreased significantly, suggesting the decreasing mucosal defense capabilities in rats with spleen de- t ficiency. Buzhongyiqitang increased their expression greatly, which suggested the prescription raise the expression of TFF1 to enhance gastric mucosal barrier and promote the reconstruction of damaged gastric mucosal, which may be one of the mechanisms of reducing gastric mucosal vulnerability of Buzhongyiqitang.

关 键 词：补中益气汤 脾虚大鼠 TFF1 

分 类 号：R285[医药卫生—中药学]