吴茱萸挥发油单次给药对小鼠肝毒性"量-时-毒"关系研究  

"Dose-time-toxicity" relationship study on hepatotoxicity caused by single dose volatile oil of Evodia Fructus to mice

作　　者：孙蓉[1] 黄伟[1] 吕丽莉[1] 

机构地区：[1]山东省中医药研究院,济南250014

出　　处：《中药药理与临床》2012年第3期55-58,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家973计划中医基础理论专项;确有疗效的有毒中药科学应用关键问题的基础研究(项目编号:2009CB522800);有毒中药的毒性与功效;证候关系的基础研究(课题编号:2009CB522802);山东省科技平台建设项目课题(课题编号:2008GG2NS02021)

摘　　要：目的:研究吴茱萸挥发油组分单次给药致小鼠肝毒性的时毒、量-毒关系。方法:取小鼠按不同时间点和不同剂量分组给药,观察小鼠死亡情况和毒性反应,检查血清谷丙转氨酶(ALT)、谷草转氨酶(AST),计算肝、脾、胸腺脏器指数,光学显微镜下观察肝组织形态变化。结果:"时-毒"关系研究表明:小鼠单次灌服1.35 ml/kg的吴茱萸挥发油后,血清ALT、AST值随时间不同造成肝损害程度也不同,均可导致肝脏指数明显升高,小鼠血清ALT、AST均在给药后6 h达到高峰;肝组织病理学检查显示,吴茱萸挥发油可在给药8～72 h内对肝组织产生明显损伤。"量-毒"关系研究表明:与正常组比较,吴茱萸挥发油不同剂量组对肝组织产生不同程度的损伤,且随着剂量增大,ALT、AST升高显著,呈剂量依赖关系。结论:小鼠单次灌服吴茱萸挥发油可造成小鼠一定的急性肝损伤,其对肝损伤程度呈现有一定的"量-时-毒"关系。To study on the time-toxicity and dose-toxicity relationships caused by single dose volatile oil of Evodia Fructus to mice. Methods： Mice were packeted according to different time and dose,/to observe the death circumstance and toxicity of mice, detect ALT , AST in serum ,calculate liver, spleen, thymus index, and observe the morphological changes of liver tissue under light microscope. Results： The ＂time-toxicity＂ relationship showed that： ALT, AST in mice serum administrated to 1.35 ml/kg volatile oil of Evodia Fructus were changed differently in different time, the liver index had significant ly increased, and ALT, AST in mice serum were peaked after 6 hours＇ad- ministration ; the histopathologieal examination of of hepatic tissue showed that the volatile oil of Evodia Fructus after 8-72 hours administra- tion to mice could produce significant liver damage. The ＂dose-toxicity＂ relationship showed that： compared with the normal group, the vola- tile oil of Evodia Fructus which had different dose could produce different degree＇s damage to hepatic tissue, the ALT, AST in serum in- creased significantly with the dose increased and have dose-dependence ralationship. Conclusion： Single dose volatile oil of Evodia Fructus to mice could produce acute liver injury, and there were certain time-toxicity and dose-toxicity relationships.

关 键 词：吴茱萸 挥发油 单次给药 肝毒性 "量-时-毒"关系 

分 类 号：R285[医药卫生—中药学]