基于UPLC-Q-TOF-MS及数据自动处理技术的刺五加提取物中刺五加苷D及其代谢产物分析  

UPLC-Q-TOF-MS and automated data analysis of eleutherosideD derived from extract of Acanthopanax senticosus Harm and its metabolites

作　　者：孙强[1] 白云[2] 包顺茹[1] 卢芳[1,3] 闫广利[1,3] 刘树民[1,3] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040 [2]黑龙江中医药大学基础医学院,哈尔滨150040 [3]北药基础与应用研究省部共建教育部重点实验室,哈尔滨150040

出　　处：《中药药理与临床》2012年第1期38-42,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家科技重大专项课题重大新药创制候选药物研究(2009ZX09103-329);国家自然科学基金青年科学基金项目(30901974);教育部"春晖计划"(Y-30);2010年教育部春晖计划项目(Z2009-1-15032)

摘　　要：目的:研究大鼠灌胃刺五加提取物后血浆和胆汁中刺五加苷D(M0)及其代谢产物。方法:大鼠灌胃给予刺五加提取物,分别采集给药后1h、1.5h、2h、4h、6h后肝门静脉血液和0～12h胆汁,采用UPLC-Q-TOF-MS技术结合Metabolynx XS软件,对大鼠血浆和胆汁中刺五加苷D及其代谢产物进行分析。结果:基于质谱裂解行为和文献报道,在大鼠血浆和胆汁中未检测到M0,但在血浆中检测到刺五加苷D代谢物(M1),在胆汁中检测到M1的进一步代谢物(M2)。其中M1和M2是作者未见文献报道的刺五加苷D的代谢产物。结论:刺五加苷D在大鼠体内是在去葡萄糖后,以葡萄糖醛酸化和去甲基化的形式被代谢。Objective： To analyze eleutherosideD（M0）and its metabolites in rat plasma and bile after oral administration Acanthopanax senticosus Harm active principle.Method： After oral administration Acanthopanax senticosus Harm active principle,rat blood samples were collected 1h,1.5h,2h,4h,6h and bile samples were collected during 0-12h.Eleutheroside D and its metabolites in rat plasma and bile were analyzed by UPLC-Q-TOF-MS coupled with Metabolynx XS.Result： M0 was not detected in rat plasma and bile,but metabolites of eleutheroside D（M1） was detected in rat plasma and further metabolites of M1（M2） was detected in rat bile based on the mass fragmentation behaviors and literature reports.M1and M2 was first reported as the metabolites of eleutheroside D.Conclusion： The results indicated that eleutheroside D can be metabolited as the form of glucuronidation and demethylation after deglucose in vivo.

关 键 词：刺五加提取物 刺五加苷D UPLC-Q-TOF-MS Metabolynx XS 代谢产物 

分 类 号：R285[医药卫生—中药学]