复肝丸对小鼠和大鼠肝纤维化模型的影响  

The effects of Fugan(复肝丸) decoction on hepatic fibrosis models in mouse and rat

作　　者：崔红燕[1] 顾伟梁[1] 马越鸣[1] 刘平[2] 刘成海[2] 朱邦贤[3] 王峥涛[4] 陶艳艳[2] 

机构地区：[1]上海中医药大学药理教研室,上海201203 [2]上海中医药大学附属曙光医院肝病所,上海201203 [3]上海中医药大学方证信息中心,上海201203 [4]上海中医药大学中药研究所,上海201203

出　　处：《中药药理与临床》2012年第1期137-141,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家科技重大专项重大新药创制(2009ZX09311-003)资助

摘　　要：目的:观察复肝丸对四氯化碳诱导的小鼠肝纤维化和二甲基亚硝胺诱导的大鼠肝纤维化的防治作用。方法:小鼠随机分为正常组、模型组、复肝丸组和培哚普利,除正常组外,其余组腹腔注射四氯化碳复制肝纤维化模型;自造模之日起各组灌胃4周。大鼠随机分为正常组、模型组、复肝丸组和培哚普利,除正常组外,其余各组腹腔注射二甲基亚硝胺4周复制肝纤维化模型;造模结束后各组灌胃4周。称重测量肝体比、脾体比,比色法测定血清谷丙转氨酶和谷草转氨酶活性,溴甲酚绿法测定血清白蛋白水平,碱消化法测定肝组织羟脯氨酸含量,HE染色观察肝组织炎性变化、天狼猩红胶原染色观察肝组织病理性胶原沉积变化。结果:与模型组相比,复肝丸10g生药/kg小鼠体重预防用药或6g/kg大鼠体重治疗用药均能降低大鼠和小鼠血清谷丙转氨酶和谷草转氨酶活性,减轻肝脏炎性病理,降低肝组织羟脯氨酸水平,改善肝组织病理性胶原沉积增生。结论:复肝丸对大鼠和小鼠肝纤维化具有防治作用。Objective： To evaluate the preventive and therapeutic effects of Fugan Decoction on liver fibrosis induced by carbon tetrachloride（CCl4） in mice and by dimethylnitrosamine（DMN） in rats.Methods： Liver fibrosis in mice was induced by intraperitoneal injection of CCl4.The rats were randomly divided into 6 groups： normal,model and Fugan Low,Fugan middle,Fugan High and N-Acetyl-L-Cysteine control group.All the mice were administrated drugs by garvage for 4 weeks from the the fist CCl4.injection day.Liver fibrosis in rats was induced by intraperitoneal injection of dimethylnitrosamine（DMN）.The rats were randomly divided into 6 groups： normal,model and Fugan Low,Fugan middle,Fugan High and Perindopril control group.The rats were administered drugs by garvage for 4 weeks after the model was established.The ratios of liver weight/body weight and spleen weight/body weight were calculated.Serum ALT,AST activity were determined by colorimetry,Alb levels were assayed by bromocresol green method;serum hepatic inflammation and collagen deposition were evaluated with HE staining and Sirius-red staining respectively;the contents of hepatic hydroxyproline（Hyp） were measured by alkali digestion method.Results： Abnormal liver function,obvious hepatic inflammation,excess collagen deposition were observed in model group both of mouse and rat,compared with those in normal group.Compared to model group,Fugan Decoction significantly down-regulated serum ALT and AST activity,alleviated liver inflammation,it also reduced the level of hepatic hydroxyproline and the pathological excess collagen deposition in liver.Conclusion： Fugan decoction has good preventive and therapeutic effects in experimental liver fibrosis.

关 键 词：复肝丸 四氯化碳 二甲基亚硝胺 肝纤维化 胶原沉积 

分 类 号：R285[医药卫生—中药学]