地黄益智方对Aβ1-40所致老年性痴呆模型大鼠细胞凋亡相关基因表达的影响  

Effects of Dihuang Yizhifang on apoptosis related gene expression in senile dementia rats induced by Aβ_(1-40)

作　　者：安红梅[1] 胡兵[1] 陈久林[2] 郁志华[2] 靳淼[1] 顾超[1] 邢三丽[2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海市中医老年医学研究所,上海200031

出　　处：《中药药理与临床》2012年第1期141-144,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：上海市教育委员会科研创新项目(10YZ58);上海市科委中药现代化项目(10DZ1971200);国家自然科学基金项目(81072789);国家973项目(2010CB530400)

摘　　要：目的:观察地黄益智方对Aβ1-40所致老年性痴呆模型大鼠脑细胞凋亡相关基因表达的影响。方法:120只大鼠随机分为空白组、模型组、地黄益智方小、中、大剂量组。采用大鼠双侧海马CA1区注射Aβ1-40复制老年性痴呆模型,造模24h后开始灌胃给药,连续4周。Real-Time PCR动态检测各组大鼠大脑皮层CDK6、Bax、Caspase-3表达变化;ELISA法动态检测Tau磷酸化。结果:Aβ1-40造模2周后CDK6、Bax、Caspase-3表达升高,Tau磷酸化升高;小、中、大剂量(1.2g、2.4g、4.8g浸膏/kg)地黄益智方可改善Aβ1-40诱导Tau磷酸化,以及CDK6、Bax、Caspase-3表达。结论:Aβ1-40可致老年性痴呆模型大鼠脑细胞Tau蛋白异常磷酸化,促使细胞周期调控基因CDK6、细胞凋亡调控基因Bax、caspase3表达,地黄益智方可以改善Aβ1-40导致的细胞周期、细胞凋亡基因表达。Objective： To observe the effects Dihuang Yizhifang（DYF） on apoptosis related gene expression in senile dementia rats induced by Aβ1-40.Method： 120 Sprague-Dawley rats are randomly divided into normal,model,low-dose,middle dose and high-dose of DYF group.Senile dementia was induced by Aβ1-40 injection in bilateral hippocampal CA1 area of these rats.After 24h of Aβ1-40 injection,rats were treated intragastrically with DYF or distilled water for 4 weeks.Expression of CDK6,Bax and Caspase-3 in cortex were detected by Real-time PCR.Tau phosphorylation was analysis by Enzyme Linked Immunosorbent Assay.Results： Expression of CDK6,Bax and caspase3,and Tau phosphorylation were up-regulated after 2 weeks of Aβ1-40 injection.Low-,middle-and high-dose of DYF（1.2g,2.4g,and 4.8g extract / kg） may ameliorate the abnormal Tau phosphorylation and expression of CDK6,Bax and caspase-3 in some extent.Conclusion： DYF may ameliorate Aβ1-40 induced Tau phosphorylation,and CDK6,Bax and Caspase-3 expression in senile dementia rats.

关 键 词：地黄益智方 老年性痴呆 细胞凋亡 基因表达 

分 类 号：R285[医药卫生—中药学]