3T3-L1前体脂肪细胞分化过程中G蛋白偶联受体48的表达研究  

G protein coupled receptor 48 gene expression analysis in differentiation of 3T3-L1 preadipocytes

作　　者：孙海燕[1] 彭永德[1] 董维平[1] 王煜非[1] 范能光[1] 刘瑞[1] 赵立[1] 丁晓颖[1] 

机构地区：[1]上海交通大学附属第一人民医院内分泌科糖尿病研究室,200800

出　　处：《中华细胞与干细胞杂志（电子版）》2012年第1期10-13,共4页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：国家自然科学基金(30800562)

摘　　要：目的 观察G蛋白偶联受体48（GPR48）、过氧化物酶体增殖体激活受体g2（PPARγ2）和CCAAT增强子结合蛋白α（C/EBPα）基因在小鼠胚胎成纤维细胞（3T3-L1）前体脂肪细胞诱导分化过程中不同时段表达水平的变化,探讨GPR48在脂肪细胞分化过程的作用。方法 体外培养3T3-L1前体脂肪细胞诱导分化为成熟脂肪细胞,在分化不同时段（第0~14天）,采用Real-timePCR技术检测脂肪细胞中GPR48、PPARγ2和C/EBPα基因信使核糖核酸（mRNA）的表达水平。结果 GPR48基因在3T3-L1前体脂肪细胞诱导分化第2天和第3天表达显著上调,差异均有统计学意义（t=4.12,P=0.015;t=6.21,P=0.003）,分化第6~14天与分化前表达无差异。PPARγ2表达在诱导分化后明显上调,分化第6天达高峰,第10~14天持续处于较高水平并趋于稳定,与诱导前期相比各时段间表达水平差异均有统计学意义（t在4.17~22.65间,P均〈0.01）。C/EBPα表达在诱导分化后明显上调,分化后第3天达高峰,第6~10天持续保持在较高水平,与诱导前期相比各时段表达水平差异均有统计学意义（t在4.38~13.87间,P均〈0.01）,第14天趋于下调,与分化前比较无差异。GPR48基因表达高峰早于PPARγ2和C/EBPα。结论 在3T3-L1脂肪细胞分化过程中PPARγ2和C/EBPα表达变化与脂肪细胞分化、脂质积聚过程相一致。GPR48基因表达高峰早于PPARγ2和C/EBPα,可能参与了脂肪细胞分化的早期过程。Objective In order to investigate the role of GPR48 in 3T3-L1 preadipocytes differentiation, we assayed expression of G protein coupled receptor 48（GPR48）, peroxisome proliferator-activated receptory2 （PPAR72） and CCAAT Enhancer binding protein CEBP α（C/ EBPα） genea during differentiation of 3T3-L1 preadipocytes. Methods 3T3-L1 cells were cultured, and induced to differentiate using 0.5 mmol/L 3-isobutyl-l-methyxanthine （IBMX）, 10μg/ ml insulin, and 1 μmol/L dexamethasone. Real-time PCR was used to evaluate gene expression during 10 days＇ differentiation period. SPSS11.0 software was used for statistic analysis. Results The expression level of GPR48 reached peak at 2 days after induction of differentiation, significantly higher than that before induction （t = 4.12, P = 0.015; t = 6.21, P = 0.003）. But the expression declined then and retuned to base level after day 6. The expression level of PPAR3,2 reached peak at day 3 after induction of differentiation, significantly higher than that before induction. The expression remained high thereafter（t = 4.17 - 22.65, P 〈 0.01）. The expression level of C/EBPα reached peak at day 3 after induction, significantly higher than that before induction （t = 4.12, P = 0.015; t = 6.21, P = 0.003）. The expression remained high thereafter（t = 4.38 - 13.87, P 〈 0.01）. The expression peak of GPR48 was earlier than those of PPAR72 and C/EBPα.Conelusion Expressions of GPR48, PPAR72, and C/EBPα mRNA were upregulated after induction of differentiation. Both PPARy2 and C/EBPα are involved in 3T3-L1 preadipocytes differentiation. GPR48 may play a role at the earlier time points after induction of differentiation.

关 键 词：G蛋白偶联受体48 3T3-L1前体脂肪细胞 细胞分化 

分 类 号：R346[医药卫生—基础医学]