绒毛状烟草钙依赖蛋白激酶基因家族分析  

Comprehensive Analysis of the Calcium-dependent Protein Kinase Gene Family in Nicotiana Tomentosiformis

作　　者：张丽[1,2] 张磊[1,2] 康乐[1,2] 王军伟 王鲁[1] 龚达平[1] 刘贯山[1] 

机构地区：[1]中国农业科学院烟草研究所,中国农业科学院烟草遗传改良与生物技术重点开放实验室,青岛266101 [2]中国农业科学院研究生院,北京100081 [3]云南烟草集团有限责任公司,云南玉溪653100

出　　处：《中国烟草科学》2012年第3期56-62,共7页Chinese Tobacco Science

基　　金：中国烟草总公司烟草基因组计划重大专项项目“烟草突变体库创制、筛选与分析”[110201101009(JY-03)];“烟草全基因组过表达和RNA干涉体系构建”(110200902036);“烟草突变体创建与功能基因组学研究”(110200701022)

摘　　要：钙依赖蛋白激酶(CDPK)是一个多基因家族,在植物的钙信号转导中具有重要作用。以拟南芥、普通烟草和烟草属其他种中已知的CDPK序列检索绒毛状烟草基因组框架图数据库,获得绒毛状烟草CDPK基因,并对其进行基因结构、GC含量、系统进化关系、主坐标和基因表达谱分析。结果表明:共分离获得25个绒毛状烟草CDPK基因,它们均具有完整的开放读码框;系统进化树和主坐标分析证实这25个CDPK分布于4个亚家族中;RT-PCR分析显示,绒毛状烟草CDPK基因家族的空间表达存在明显的差异性。此为进一步阐明CDPK基因家族在烟草的钙信号转导过程中的功能及分子机制奠定了重要基础。CDPK are a multigene family, and play important roles in plant calcium signal transduction. The CDPKs that consist of Arabidopsis thaliana, N. tabacum and other species in tobacco genus were used to search the N. tomentosiformis genome databases. Then, the CDPK genes were isolated from N. tomentosiformis. The objectives of this investigation were to further study CDPK sequence characteristics, evolutionary relationship and gene expression, The results indicated that full-length genomic DNA sequences of 25 CDPK genes were obtained from N. tomentosiformis. Phylogenetic and principal coordinate analysis divided the 25 CDPKs into 4 subfamilies. The results of reverse transcription-PCR （RT-PCR） showed that the expression patterns of the 25 CDPK genes were significantly different. This study provides an important foundation for further functional dissection of the tobacco CDPK gene family.

关 键 词：绒毛状烟草 烟草 CDPK基因家族 进化树 基因表达 

分 类 号：TS413[农业科学—烟草工业]