烟田杂草小蓟锈病菌遗传多态性的RAPD分析  

The Analysis of Puccinia obtegens Genetic Polymorphic

作　　者：张峻铨[1] 时焦[1] 韦建玉[2] 孟坤[1] 

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]广西中烟工业有限责任公司,南宁530001

出　　处：《中国烟草科学》2012年第3期63-67,共5页Chinese Tobacco Science

基　　金：中国农业科学院院长基金项目(200912)

摘　　要：从50条Random Amplified Polymorphic DNAs(RAPD)随机引物中选取多态扩增性强的15条引物,对蓟柄锈菌(Puccinia obtegens)的22个样品和3个大蓟上采集的锈病菌样品基因组DNA进行扩增,并构建指纹图谱。25个样品包括来自不同地区的9个夏孢子样品,不同孢子类型的6个样品,同一地点不同采集时间的7个夏孢子样品和3个大蓟上采集的样品。结果显示,扩增共产生DNA标记谱带204条,多态性谱带数为160条,多态检测率为78.4%。利用SAS统计分析软件对样品的PCR结果进行了UPGMA(非加权类平均法)聚类分析。结果显示,供试3组样品可分别划分为Ⅰ、Ⅱ、Ⅲ3个聚类组。研究结果证明,供试样品具有丰富的遗传多态性,且样品间的亲缘关系与来源地、孢子类型和采集时间有一定的相关性。Fifteen of Random Amplified Polymorphic DNAs （RAPD） primers with strong amplitication of polymophic were selected from 50 of random primers. The selected 15 of primers were used to amplify genome DNAs; of 22 of Puccinia obtegens samples and 3 of samples from Cephalanoplos setosum （Willd） Kitam, finally construct fingerprint picl：ures. The 25 samples consist of 9 urediospore samples collected from different locations, 6 samples with different spore types, 7 urediospore samples from same location collected at different dates and 3 samples from Cephalanoplos setosum （Willd） Kitam. The results showed that 204 mark bands were produced, 160 were polymorphic ones, polymorphism detection rate was 78.4%. The 3 groups of tested samples were divided into 3 cluster groups by SAS cluster analysis, respectively. The results proved that the tested samples had plenty of genetic polymorphisms, and further, their genetic relationship was related to origins, hosts, spore types and collecting times.

关 键 词：小蓟 蓟柄锈菌 遗传多态性 RAPD 烟草 

分 类 号：S451[农业科学—植物保护]