高糖高脂联合低剂量STZ诱导版纳微型猪2型糖尿病动物模型的建立  

Establishment of Model of Type 2 Diabetes in Chinese Banna Miniature Pigs Induced by High Sucrose/High Fat Diet and STZ

作　　者：肖国华[1,2] 张素君[3,4] 余坚[4] 高天舒[1] 王月婷[3,2] 张亚丽[3,2] 王宗保[3,4,2] 

机构地区：[1]南华大学医学院肿瘤研究所,湖南衡阳421001 [2]南华大学药学与生命科学学院 [3]南华大学动脉硬化学湖南省重点实验室 [4]南华大学实验动物部

出　　处：《中南医学科学杂志》2012年第4期351-356,共6页Medical Science Journal of Central South China

基　　金：湖南省科技厅重点项目(2010TT1008);湖南省科技厅科研项目(2009SKJ17);湖南省高校科技创新团队支持计划资助

摘　　要：目的用云南版纳微型猪建立一种新的2型糖尿病模型,探讨高糖高脂联合链脲佐菌素(STZ)对糖、脂代谢及肝、肾、胰组织结构的影响。方法高糖高脂联合低剂量STZ诱导云南版纳微型猪2型糖尿病模型,观察其血糖、胰岛素、血脂的变化,并对其肝、肾、胰组织结构的病理改变进行研究。结果喂养12月后,与正常对照组比较,模型组空腹血糖值明显升高,血清总胆固醇、甘油三酯水平升高(P<0.05),胰岛β细胞受损,脂肪变性明显,胰岛数量明显减少,胰岛内细胞数目亦减少,胞核浓染、缩小,红染加深;肝脏脂肪样变性,肝细胞索排列紊乱,肝细胞肿胀呈气球样变,肾小球肥大,硬化,肾小球内细胞增多,空泡变性。结论高糖高脂联合STZ可诱导版纳微型猪发生胰岛素抵抗、糖尿病,既表现2型糖尿病的特征,又表现1型糖尿病的特征,成功模拟了人类2型糖尿病的发病过程。Objective To establish a new animal model of the human type 2 diabetes mellitus and investigate the effects of high sucrose/high fat diet and STZ on glucose,lipid metabolism and organizational structure of liver,kidney,pancreas in Chinese banna lminiature pigs. Methods Minipigs were induced by high sucrose/high fat diet and STZ and the levels of fasting blood glucose, insulin, total cholesterol, triglyeerides were measured. Organizational structure of liver, kidney and pancreas were observed by Hematoxylin eosin staining and scanning electron photomicrographs in the minipigs. Results The fasting plasma glucose, triglyceride and total cholesterol of model group increased significantly compared to that of control group at the end of 12 months （P 〈 0.05 ）. Injured pancreatic [3-cells were observed, and the pancreatic islets from the model group became atrophied, with loss in number of the islets and cells within them. The nucleus of islet cells were small and deep - dyed. There was an abundance of large vacuolations and arrangement of liver cell cord in disorder in cytoplasm. Hepatic steatosis, as well as glomerular hypertrophy and glomerulosclerosis, could be observed in the minipigs of model group. Conclusions Our study successfully established a new animal model that feeding minipigs with high sucrose/high fat diet and STZ without adding dietary cholesterol which can induce insulin resistance and severe diabetes. It showed the characteristic of not only type 2 diabetes ,but also type 1 diabetes. It simulated human type 2 diabetes pathogenesis.

关 键 词：链脲佐菌素 高糖高脂饲料 2型糖尿病 动物模型 版纳微型猪 

分 类 号：R587.1[医药卫生—内分泌]