乙醇-水反相高效液相色谱法在中药及中成药分析中的应用  

The Application of Ethanol-Water System RP-HPLC Method in Analyzing Chinese Traditional Medicines and Medicinal Preparations

作　　者：付春梅[1] 李章万[1] 刘三康[1] 陈聪[1] 

机构地区：[1]华西医科大学药学院,99届研究生成都610041

出　　处：《药物分析杂志》2000年第1期37-41,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的： 建立用无毒的乙醇作为反相高效液相色谱流动相溶剂分析中药及中成药有效成分的方法。方法：用乙醇－ 水系统作反相高效液相色谱流动相测定中药及中成药的主要有效成分， 并与甲醇－ 水或乙腈－ 水系统的测定结果进行比较。结果：２ 种色谱系统均达到基线分离， 测得含量基本一致， 乙醇系统与甲醇系统或乙腈系统相比， 测定相对偏差＜ ±３％ （ｎ ＝ ３）。结论： 选择合适的柱温等色谱条件， 乙醇可用作反相高效液相色谱流动相分析中药及中成药中有效成分， 安全、准确。Objective:To establish RP-HPLCs using ethanol as mobile phase solvent for analyzing Chinese traditionnal medicines and Chinese traditionnal medicinal preparations.Methods:The main components of some Chinese medicines and Chinese medicinal preparations were separated and measured by RP-HPLC using ethanol-water as mobile phase .Results:The results were compared with those obtained with methanol-water or acetonitrile-water. The relative standard deviations were small than 3%( n =3).Conclusions:Ethanol can be used as RP-HPLC mobile phase solvent by adjusting the column temperature moderatly. The method is safe and accurate.

关 键 词：高效液相色谱 中药 中成药 乙醇 水 

分 类 号：R284.4[医药卫生—中药学] R286.0[医药卫生—中医学]