斑蝥素与去甲斑蝥素对昆虫细胞Sf9抑制作用比较  

Comparisons of inhibition between cantharidin and norcantharidin on Sf9 cells

作　　者：周晗颖 杨宝东[1] 张民照[1] 王进忠[1] 张爱环[1] 张志勇[1] 

机构地区：[1]农业应用新技术北京市重点实验室,北京农学院植物科学技术学院,北京102206

出　　处：《环境昆虫学报》2012年第2期154-160,共7页Journal of Environmental Entomology

基　　金：国家自然科学基金项目(30971938);北京市教委科技创新平台项目(PXM2008_014207_055164);北京市高校人才强教计划项目(PHR-201107135);国家科技支撑计划(2012BAD19B0b)

摘　　要：为探索斑蝥素的类似物去甲斑蝥素对昆虫的毒杀机理,本研究利用草地贪夜蛾Spodoptera frugiperda卵巢细胞系Sf9细胞作为实验材料,采用CCK-8法和AO/EB荧光染色法比较了斑蝥素(CTD)和去甲斑蝥素(NCTD)对细胞的增殖抑制和抑制方式。结果显示,3.125μg/mL的CTD处理细胞抑制率6h为20.00±1.10%,24h为66.33±0.81%;50μg/mL的CTD处理细胞抑制率6h为42.69±6.34%,24h为74.60±1.51%。3.125μg/mL的NCTD处理细胞抑制率6h为10.03±1.72%,24h为21.75±6.22%;50μg/mL的NCTD处理细胞抑制率6h为40.79±1.32%,24h为66.08±3.32%。结果表明,CTD与NCTD均能有效抑制Sf9细胞增殖和诱导细胞凋亡,且抑制率呈现出时间、剂量依赖性,较高剂量NCTD可以替代CTD达到相同效果。To investigate the mechanism of NCTD＇s toxic effect on Sf9 cells,CCK-8 assay and AO/EB fluorescent dyes were used to detect the inhibition rates and the way of function.There results showed that,treated with CTD of 3.125 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 20.00 ± 1.10% and 66.33 ± 0.81% ;treated with CTD of 50 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 42.69 ± 6.34% and 74.60 ± 1.51%.Treated with NCTD of 3.125 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 10.03 ± 1.72% and 21.75 ± 6.22% ;treated with NCTD of 50 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 40.79 ± 1.32% and 66.08 ± 3.32%.It was indicated that,both of CTD and NCTD could effectively inhibit cell＇s proliferation in concentration-dependent and time-dependent,higher concentration of NCTD could replace lower concentration of CTD to reach similar inhibition rates,and NCTD could induce apoptosis significantly as well as CTD.

关 键 词：斑蝥素 去甲斑蝥素 Sf9 增殖抑制 细胞凋亡 

分 类 号：Q966[生物学—昆虫学]