重金属胁迫培养对沼泽红假单胞菌生长的影响  

The influence of culturing under the heavy metals stress on the growth of Rhodopseudomonas palustris

作　　者：全亚玲[1] 欧小兵[1] 戴静[1] 李涵依[1] 王燕[1] 郝葆青[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《西南民族大学学报（自然科学版）》2012年第4期570-573,共4页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：成都市横向项目"光合菌综合开发及应用研究"

摘　　要：目的:观察和检测重金属胁迫下沼泽红假单胞菌生长和生理特性,为进一步探索沼泽红假单胞菌在重金属污染防治方面的应用提供一定的实验数据.方法:将沼泽红假单胞菌分别接种到含有五种不同重金属离子{镉(Cd2+)、汞(Hg2+)、铜(Cu2+)、钴(Co2+)、铅(Pb2+)}不同浓度的培养基(即胁迫下)和对照培养基中进行厌氧光照培养,分别测定其菌株生长量.结果:微量的Cd2+、Co2+、Pb2+对沼泽红假单胞菌的生长影响不大,但当浓度达到1×10-5mol·L-1时,其生长受阻;而微量的Hg2+、Cu2+则抑制沼泽红假单胞菌的生长,当Hg2+浓度达到1×10-5mol·L-1,Cu2+浓度达到1×10-6mol·L-1时,对沼泽红假单胞菌生长抑制作用显著.结论:本实验的沼泽红假单胞菌在五种重金属胁迫条件下均能生长,且对五种重金属耐受性大小顺序依次为:Co2+>Pb2+>Cd2+>Hg2+>Cu2+.Objective： To observe and test the growth properties of Rhodopseudomonas＇palustris under the heavy metals, and the experimental data are obtained for exploring further the application of Rhodopseudomonas palustris in anti-pollution of the heavy metal. Methods： Rhodopseudomonas palustris is grown anaerobically in each medium containing different concentrations of heavy metal ions （Cd2＋, Hg2＋, Cu2＋, Co2＋ and Pb2＋） and in a control medium, and the total number of bacterial colony is measured separately. Results： Micro-ion concentrations of Cd2＋, Co2＋, Pb2＋ do not influence the growth of Rhodopseudomonas palustris until the concentration at 1 × 10^-5mol.L^-1. And it shows that the growth of Rhodopseudomonas palustris can be inhibited by the micro-ion concentrations of Hg2＋ and Cu2＋, and it will be remarkably influenced when it reaches the ion-concentration at 1× 105mol.L^-1for Hg2＋ and at 1× 10-6mol.L-I for Cu2＋. Conclusion： Rhodopseudomonas palustris can grow under all of the five heavy metals stress, and the tolerance to the five heavy metals for Rhodopseudornonas palustris is in turn as follows： Co2＋〉 pb2＋〉Cd2＋〉Hg2＋〉Cu2＋.

关 键 词：沼泽红假单胞菌:重金属离子:胁迫:污染 生长特性 

分 类 号：S182[农业科学—农业基础科学]