高效液相色谱法测定逍遥丸(水丸)中甘草苷含量  

Determination of Liquoritin in Xiaoyao Pills by HPLC

作　　者：韩慧琴 王晓青[2] 尹贻珍 潘瑞玲[3] 董存元[4] 

机构地区：[1]河北省张家口市药品检验所,河北张家口075000 [2]内蒙古医学院药学院,内蒙古呼和浩特010059 [3]内蒙古医学院第二附属医院,内蒙古呼和浩特010059 [4]河北北方学院第三附属医院,河北张家口075000

出　　处：《中国药业》2012年第13期21-22,共2页China Pharmaceuticals

摘　　要：目的建立测定逍遥丸中甘草苷含量的高效液相色谱法。方法色谱柱为Agilent C18柱(150 mm×4.6 mm,5μm),以乙腈-0.05%醋酸溶液(13∶87)为流动相,流速为1.0 mL/min,检测波长为276 nm。结果甘草苷进样量线性范围为0.008～0.4μg,r=0.999 9,平均回收率为99.79%,RSD=0.05%(n=6)。结论该方法操作简便、准确、灵敏、具有良好的耐用性,可作为逍遥丸中甘草苷的定量分析方法。Objective To establish a method for HPLC determination of liquoritin in Xiaoyao Pills.Methods Agilent（150 mm×4.6 mm,5 μm）column as fixed phase and acetonitrile-0.05%acetic acid solution（13 ∶87）as mobile phase was adopted.The flow rate of 1.0 mL/min and the detective wavelength was 276 nm.Results The linear range was 0.008-0.4 μg（r=0.999 9）,the average recovery of liquoritin was 99.79%,RSD=0.05%（n=6）.Conclusion The method is simple,accurate and sensitive.It is adoptable for quantitative analysis of liquoritin in Xiaoyao pills.

关 键 词：逍遥丸 甘草苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]