γ-聚谷氨酸合成酶系PgsBCA结构的生物信息学分析  

Sequence Analysis of PgsBCA of Poly-γ-glutamate Synthetase System

作　　者：姚文娟[1] 范文俊[1] 许小乐[1] 邓小昭[2] 张伟[1] 

机构地区：[1]南通大学医学院,江苏南通226001 [2]南京军区军事医学研究所,江苏南京210002

出　　处：《南通大学学报（自然科学版）》2012年第2期41-46,共6页Journal of Nantong University(Natural Science Edition)　

基　　金：江苏高校优势学科建设工程资助项目;南通市科技计划项目(AS2011020)

摘　　要：利用ProtParam、TopPred、PredictProtein、PSORT-B Prediction、SWISS-MODEL等软件分别分析蛋白质的理化性质、跨膜区、二级结构、亚细胞定位、三维结构.结果显示:PgsB是亲水不稳定蛋白,通过豆蔻酰锚钩锚定于质膜上,催化作用需与ATP结合提供能量;PgsC是疏水稳定蛋白,通过4个跨膜区和多个豆蔻酰锚钩定位于质膜,具有酰胺化位点;PgsA是亲水稳定蛋白,通过N端一个跨膜区和豆蔻酰锚钩结合于质膜,具有多种磷酸化位点.说明γ-聚谷氨酸(Polyγ-glutamic acid,γ-PGA)合成酶系3个组分蛋白形成复合物定位于质膜上,其中PgsB在胞内催化γ-PGA合成,PgsC固定于质膜,连接PgsB和PgsA组分,PgsA在胞外负责γ-PGA的运输.通过对γ-PGA合成酶系各组分蛋白结构的分析,为日后在谷氨酸高产菌株中的表达奠定了基础.The physico-chemical properties,trans-membrane domains,secondary structures,sub-cellular localization and spatial structures are studied by ProtParam,TopPred,PredictProtein,PSORT-B Prediction and SWISS-MODEL,respectively.The results show that PgsB is a protein of hydrophilia and instability,and localizes to the membrane by myristoyl anchors.The catalytic action of PgsB requires combination of ATP to provide energy;PgsC is hydrophobic and stable,and localizes to the membrane by four trans-membrane domains and myristoyl anchors.PgsC has an amidation motif.PgsA is hydrophilic and stable,and localizes to the membrane by a trans-membrane domain in N terminus and myristoyl anchors.PgsC has many phosphorylation motifs.The results demonstrate that the compound of the three constituents from γ-PGA synthetase system is localized to the membrane.PgsB catalyzes γ-PGA synthesis.PgsC links PgsB and PgsA in the membrane.PgsA transports γ-PGA outside the cell.This study analyzes the structures of constituents from poly-γ-glutamate（γ-PGA） synthetase system in order to benefit expression of the synthetase system in the glutamate-producing strain.

关 键 词：Γ-聚谷氨酸 生物信息学 PgsBCA 

分 类 号：R966[医药卫生—药理学]