高效毛细管电泳法测定黄芪中黄芪甲苷的含量  

Determination of astragaloside Ⅳ in Astragali Radix preparations by high efficiency capillary chromatography

作　　者：杨天鸣[1] 裴婷[1] 付海燕[1] 黄灿[1] 王云英[1] 

机构地区：[1]中南民族大学药学院,湖北武汉430074

出　　处：《武汉工程大学学报》2012年第6期1-3,共3页Journal of Wuhan Institute of Technology

基　　金：国家"十二五"科技支撑计划资助项目(2012BAI27B00);中央高校科研专项资助重点项目(CZZ10005);湖南大学化学生物传感与计量学国家重点实验开放基金(201111)

摘　　要：为了建立能分离测定黄芪甲苷含量的胶束毛细管电泳法,采用美国Beckman公司P/ACEMDQ毛细管电泳系统,二极管阵列检测器,未涂层弹性石英毛细管(75μm×60 cm,有效分离长度50 cm),检测波长203 nm,进样时间5 s,分离电压20 kV,温度25℃,分离时间15 min,缓冲体系:20 mmol/L磷酸二氢钠—40mmol/L硼砂—30 mmol/LSDS—10%乙腈(pH=8.5).结果表明:黄芪甲苷在0.035～0.7 mg/mL与峰面积线性关系良好(r=0.999 2),黄芪甲苷的回收率为98.30%.该法简单、快速、准确、消耗少、重现性好,可用于黄芪药材的质量控制.A rapid and simple method was used to determine Astragaloside Ⅳ in Radix astragali by micellar electrokinelic capillary chromalonraphic（MECC）. Separations were carried out in a fused -silica capillary tube with peak detection at 203 nm. Operating voltage was 20 kV and temperature was maintained at 25 ℃ while injection was 5 s and Separation time was 15 min. The buffer was composed of 20 mmol/L Sodium Dihydrogen Phosphate ＋ 40 mmol/L Borax ＋ 30 mmol/L SDS ＋ 10% Acetonitrile （pH = 8. 5）. Under the chosen condition, the linear concentration of Astragaloside 1V ranged from 0. 035 to 0. 700 mg/mL（r = 0. 999 2）. The method was used for analysis of Astragaloside Ⅳ with recovery of 98. 30%. This method is simple, good repeatability and rapidity and could be used as quality control for Radix astragali.

关 键 词：黄芪 黄芪甲苷 高效毛细管电泳 胶束毛细管电泳法 

分 类 号：O657.33[理学—分析化学]