1株堆肥耐低温纤维素降解菌的筛选、鉴定及生长特性的初步研究  

Screening,identification and growth of a cold-adapted cellulose-decomposing bacterium for composting

作　　者：尚晓瑛[1] 程旭艳[1] 霍培书[1] 李季[1] 

机构地区：[1]中国农业大学资源与环境学院,北京100193

出　　处：《华中农业大学学报》2012年第5期558-562,共5页Journal of Huazhong Agricultural University

基　　金：国家"十二五"科技支撑计划项目(2012BAD14B01);省部产学研结合项目(粤财教[2011]366专项)

摘　　要：为增加用于堆肥的耐低温纤维素分解菌的资源,通过微生物筛选实验及纤维素酶活力测定实验,从黑龙江双峰林场采集的腐殖土样中筛选得到8株耐低温纤维素降解菌,其中菌株B6-15的纤维素酶活性最高,为24.94U/mL,且该菌在初始pH 7.0,NaCl质量分数0.25%,20℃环境中培养时生长量最大。采用形态学、生理生化特征及16SrDNA核酸序列分析鉴定的方法,初步确定该菌属于假单胞菌属(Pseudomonas sp.)。对菌株B6-15所产纤维素酶的热稳定性进行研究,结果显示在10℃存放后仍能保持54.43%的酶活性,表明该耐低温菌所产的纤维素酶为低温酶。Through the screening experiment and the cellulase activity measurement experiment, eight cold-adapted cellulose-decomposing bacteria have been isolated from forest humus at Heilongjiang Province for increasing the resources of cold-adapted cellulose-decomposing bacteria for composting. Among the eight bacteria,the strain B6-15 had the highest cellulase activity of 24. 94 U/mL. And when the strain B6-15 was cultivated at pH 7. 0,0. 25% of NaCI concentration, it could get the maximum growth. Strain B6-15 was identified as a Pseudornonas sp. by its morphological, the physiological and bio- chemical properties and 16S rDNA sequence analysis methods. Then the thermal stability of cellulase produced by strain B6-15 was examined and the result showed that the cellulase was a kind of cold active enzyme and had 54. 430% residual activity at 10 ℃.

关 键 词：耐低温菌 纤维素降解 假单胞菌属 筛选 生长特性 

分 类 号：Q93-33[生物学—微生物学]