改良体外精液优选技术的实验研究  

Modified in vitro sperm selection technology

作　　者：石汉振[1] 冯妙芙[1] 邓雪梅[2] 文任乾 

机构地区：[1]广东省中医院检验科,广州510120 [2]广东省中医院生殖医学科,广州510120 [3]广东省计划生育专科医院,广州510120

出　　处：《国际医药卫生导报》2012年第13期1857-1859,共3页International Medicine and Health Guidance News

摘　　要：目的为了能最大程度获得高质量和足够数量的精子，根据《WHO人类精液检验与处理实验室手册》第五版要求对体外精液优选技术进行了改进，以期为患者提供一种更安全、可靠、高效的精液优选方法。方法收集2011年1至9月广东省中医院生殖医学科共计68对不育夫妇的98份精液标本，采用改良前后的精液优选方法（密度梯度法和上游法）分别进行精液优选处理。优选前后使用改良Neubauer血细胞计数器进行精子浓度分析，西班牙SCA精子分析仪测定精子活动力，改良巴氏染色进行精子形态学分析。结果改良后两种方法回收的精液总活动精子数、前向运动（a＋b）精子总数、回收率有所提高（P〈0．05）；其精子浓度、正常形态精子百分率未有明显变化（P〉0．05）。优选前后比较正常形态精子有显著提高（P〈0．01）。结论改良后精液优选技术能提高患者的前向运动精子总数及回收率，有效提高不育夫妇受孕的机率。Objective To improve in vitro sperm selection technology basing on WHO lab mamual for Examination and Process of Human Semen （ fifth edition ） to offer patients with a safer, more reliable and effective sperm selection procedure. Methods 98 specimens from 68 sterility couples were collected. The specimen were detected by the old selection technology and the modified technology （ Direct swim-up and Discontinuous density-gradients ）. Neubauer Blood Cell counter was used to detect the sperm concentration. Spain SCA-CASA was used to assess sperm motility and Papanicolaou Staining was used to detect sperm morphology, ltesults The forward sperm quantity and recovery im- proved after modification （ P〈 0.05 ）. The sperm concentration and normal forms of sperm did not change after modification （ P 〉 0.05 ）. The count of sperm with normal forms increased after selection （ P 〈 0.01 ）. Conclusions Modified sperm selection technology can improve the quantity of forward sperm and the recovery rate. It can increase the chance of conception in sterility couples.

关 键 词：精液优选 改良 男性不育 

分 类 号：R698.2[医药卫生—泌尿科学]