高效液相色谱法测定根痛平滴丸中芍药苷和葛根素的含量  

High performance liquid chromatographic method for determination of contents of paeoniflorin and puerarin in root pain ping pills

作　　者：庞惠民[1] 郁相云[1] 钟建华[1] 

机构地区：[1]深圳市龙岗中心医院,518116

出　　处：《国际医药卫生导报》2012年第13期1947-1948,共2页International Medicine and Health Guidance News

摘　　要：目的通过采用高效液相色谱法来测定根痛平滴丸中芍药苷和葛根素的含量。方法应用AgelaTMC18色谱柱，水-甲醇-乙腈为流动相，流速为0．96ml／min，柱温为30％，250nm的检测波长，测定根痛平滴丸中芍药苷和葛根素的含量并作相关分析。结果本品中每丸含芍药苷不小于0．80mg，葛根素不小于0．28mg，芍药苷于59—1150mg／L，葛根素于9．0—180mg／L的范围内线性关系好。结论采用高效液相色谱法测定药物成分含量，操作简单、结果精确，回收率良好和重现性满意，用于根痛平滴丸质量控制可获得良好效果。Objective To detect the contents of paeoniflorin and puerarin in root pain ping pills by using high performance liquid chromatography. Methods Agela TMC 18 column was used and water methanol acetonitrile was used as mobile phase, with a flow rate of 0.96 ml / min, column temperature of 30℃, and detection wavelength of 250 nm, to determine the contents of paeoniflorin and puerarin in root pain ping pills ; and the related analysis was conducted. Results Paeoniflorin was not less than 0.80 mg and puerarin was not less than 0.28 mg in each pill. Paeoriflorin in 59 ~ 1150 mg / L and puerarin in 9~180 mg / L had a good linear relation. Conclusions High performance liquid chromatography for detecting drug content is simple to operate and accurate and has good reproducibility and a satisfied recovery rate. It has a good effect for quality control of root pain ping pillst.

关 键 词：根痛平滴丸 芍药苷 葛根素 高效液相色谱法 含量 

分 类 号：O657.72[理学—分析化学] R286[理学—化学]