LC-MS/MS法快速分析不同吸烟量人体唾液尼古丁及其代谢物水平  

A Fast Method for the Simultaneous Determination of Salivary Nicotine and Its Metabolites at Different Smoking Levels by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：王晔[1] 周宛虹[1] 金永明[1] 郑赛晶[1] 

机构地区：[1]上海烟草集团有限责任公司技术中心,上海200082

出　　处：《分析测试学报》2012年第7期797-803,共7页Journal of Instrumental Analysis

摘　　要：采用Salivette采样管收集人体唾液,以氘代尼古丁、氘代可的宁以及3-羟基-氘代可的宁为内标,在LC-MS/MS的大气压化学电离(APCI)离子化模式下,建立了同时测定吸烟者唾液中尼古丁及其代谢物的分析方法。该法前处理简单,色谱运行时间不足1 min,尼古丁及其代谢产物的加标回收率为94%～104%,相对标准偏差为0.29%～4.0%,线性相关系数均大于0.998。对不同吸烟量志愿者的唾液样本进行了分析,并根据唾液中尼古丁及其代谢物的含量总和,使用ada-boost算法对不同吸烟量进行分类预测,准确度可达76%。实验结果表明,可的宁在唾液与血液(r=0.92)、唾液与尿液(r=0.84)中的浓度均具有一定的相关性,且半衰期较长,适合作为评估烟气暴露程度以及尼古丁摄入量的生化指标。A fast, simple method for the determination of nicotine and its metabolites in human saliva was developed and validated by liquid chromatography -tandem mass spectrometry （LC -MS/MS）. Human saliva was collected with Salivette collection kit and the quantification of analytes was a- chieved by using nicotinemethyl-d3, cotinine-methyl-d3 and trans-3＇-hydroxycotinine-methyl-d3 as in- ternal standard under atmosphere pressure chemical ionization（APCI） mode. The pretreatment of this method was simple and fast, and all analytes were eluted within 1 rain. The relative standard devia- tions（RSDs） for nicotine and its two metabolites were between 0.29% and 4.0% and the recovery ranged from 94% to 104%. The linear correlations were above 0. 998. Saliva, plasma and urine samples from 70 volunteers were collected and quantified by the described procedure. The classifica- tion of different smoking amount was also made using ada - boost based on the total concentration of salivary nicotine and its metabolites by a predicting accuracy of 76%. A good correlation of cotinine levels bewteen saliva and plasma （r = 0. 92）, saliva and urine （ r = 0. 84） was found. The results showed that the cotinine in saliva has a longer half-life and becomes the most desirable biomarker for the evaluation of smoking exposure and nicotine uptake.

关 键 词：LC-MS/MS 唾液 尼古丁 可的宁 半衰期 生化标记物 

分 类 号：O657.63[理学—分析化学] TS411[理学—化学]