抗噬菌体色氨酸酶基因工程菌的筛选  

Screening of Phage-Resistant Tryptophanase Genetic Engineering Strains

作　　者：梅运军[1] 牟艳华 石德太 胡纯[1] 王文清[1] 

机构地区：[1]武汉工业学院化学与环境工程学院,武汉430023 [2]湖北省恩施州科技局,湖北恩施445000

出　　处：《湖北农业科学》2012年第12期2596-2598,共3页Hubei Agricultural Sciences

摘　　要：利用自发突变、紫外诱变、协同进化、紫外耦合协同进化4种方法对色氨酸酶基因工程菌WD0801进行了抗噬菌体筛选。结果表明紫外耦合协同进化法具有较好的筛选效果,筛选的42株稳定遗传的抗性菌株中有23株的长势高于出发株,11株的酶活优于出发株。4 methods,spontaneous mutation,UV mutagenesis,co-evolution,UV coupled co-evolution,were employed to screen Escherichia coli tryptophanase genetic engineering strains.The results showed that the screening efficiency of UV-coupled co-evolution method was the best.Among the 42 stable phage-resistant strains screened out,the growth vigor of 23 strains,and enzyme activity of 11 strains were higher than the original strain.

关 键 词：色氨酸酶基因工程菌 抗噬菌体 筛选 

分 类 号：Q939.97[生物学—微生物学]