青叶胆中齐墩果酸的制备工艺及其纯度测定  

Preparation of oleanolic acid from Swertia mileensis and its purity determination

作　　者：李开泉[1,2] 曾晓敏[3] 

机构地区：[1]江西省天然药物活性成分研究重点实验室,宜春336000 [2]宜春学院化学与生物工程学院,宜春336000 [3]宜春学院教育科技园,宜春336000

出　　处：《生物加工过程》2012年第4期38-41,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2002AA2Z3217)

摘　　要：研究青叶胆中齐墩果酸的提取工艺及其结构鉴定。用体积分数95%乙醇回流提取2次,每次1.5 h,减压回收乙醇后用水洗涤得沉降物。用YCXY-2号除杂剂处理后,加活性炭脱色30 min 2次,经凝析分离、纯化精制得齐墩果酸产品。采用理化常数测定和薄层色谱、红外光谱、质谱及核磁共振光谱鉴定试样的化学结构,确定实验所得产品为齐墩果酸,而齐墩果酸为青叶胆的主要化学成分。The extraction process of oleanolic acid from Swertia mileensis was investigated and its structure was identified.Samples of Swertiae mileensis Herba were refluxed with 95% ethanol for 2 times,each time for 1.5 h.After treated with YCXY-2 and activated carbon for 30 min 2 times,the condensate was separated and the refined oleanolic acid was obtained.The oleanolic acid was confirmed by physicochemical constant determination,infrared spectrum analysis,mass spectrometric analysis,and NMR spectrum analysis.

关 键 词：提取分离 齐墩果酸 青叶胆 

分 类 号：TQ464.2[化学工程—制药化工]