包衣微丸型碱性蛋白酶的制备  

Preparation of coated granule alkaline protease

作　　者：马俊孝 张赞剑 史衍鲁 卢彦梅 刘伟 

机构地区：[1]潍坊康地恩生物科技有限公司,高密261500

出　　处：《生物加工过程》2012年第4期59-63,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2012AA10A509)

摘　　要：开发了一种包衣微丸型碱性蛋白酶的制备工艺,结果表明:30 L发酵中罐发酵50 h比酶活可达4.26×104U/mL,发酵液经絮凝处理、板框压滤、膜浓缩后可制成酶活达300 000 U/mL的酶浓缩液。经流化后制得含酶颗粒,再包裹薄层后,得包衣微丸型碱性蛋白酶。对制备的包衣微丸型碱性蛋白酶的稳定性、去污效果等指标进行了评估。制备的包衣微丸型碱性蛋白酶产品在严苛条件下的稳定性与国外产品(Savinase 8.0T、PuraFast 2000HS)相当,产品暴露在37℃、75%湿度下8周后仍可保持74%的酶活力,产品的去污效果优于国外产品。制备的包衣微丸型碱性蛋白酶颗粒大小均匀,流动性和分散性好,对外界高温、高湿等不良环境具有很强的抵抗能力,适于工业化生产。A process was developed for the preparing of coated granule alkaline protease.The method for preparing enzyme solution included fermentation,flocculation sedimentation,filtration,and ultrafiltration by membrane.The final products were prepared by producing a core containing enzymes and spraying the coating layer onto the core in a fluid-bed.The stability and detergency of the coated granule were investigated by accelerated experiments,and the characteristics of products were evaluated.The main results were as follows：the activity of protease was about 4.26×104 U/mL after cultivating for 50 h in 30 L fermentor：The granule alkaline protease was characterized by a uniform particle size distribution,a high rate of finished products,and a free-flowing granulate（300 000 U/mL）.When exposed at 37 ℃ and 75% relative humidity for 8 weeks,the coated granule retained 74% of the original activity.Furthermore,the detergency was better than that of a commercial product.

关 键 词：碱性蛋白酶 包衣 去污力 稳定性 

分 类 号：TQ649[化学工程—精细化工]