O^6-甲基鸟嘌呤-DNA甲基转移酶在脑胶质瘤的表达及其临床意义  

Expressions of MGMT in Human Glioma and Its Clinical Significances

作　　者：潘勤[1] 李炜[1] 佟建州 贺建辉[1] 吴红记[1] 甘宁[1] 贾振岭[1] 

机构地区：[1]河北省保定市第一中心医院神经外科,河北保定071000

出　　处：《中国药业》2012年第14期35-37,共3页China Pharmaceuticals

摘　　要：目的探讨O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)在脑胶质瘤细胞中的表达及其与患者生存时间的关系,以判断患者的预后,并指导胶质瘤术后化学治疗药物的合理选择。方法选取手术切除的59例脑胶质瘤标本,经10%甲醛固定,常规脱水,石蜡包埋;每例标本以5μm厚度连续切片,作HE染色确定病理组织类型;对患者的生存期进行调查;用免疫组化EnVision法检测脑胶质瘤细胞中MGMT的表达情况;免疫反应染色结果按照Krajewska法进行半定量分析;所有统计分析用SPSS17.0统计软件进行,采用卡方检验以及将MGMT表达与患者生存期绘制成Kaplan-Meier生存曲线,并进行log-rank检验和分析。结果 MGMT在脑胶质瘤的表达存在异质性;MGMT在不同病理级别的脑胶质瘤细胞中表达阳性率不同,差异无统计学意义(P>0.05);MGMT表达阳性患者生存时间低于表达阴性患者,差异有统计学意义(P<0.05)。结论MGMT在脑胶质瘤的表达与肿瘤病理级别无关,MGMT的表达可作为判断预后的指标。Objective To investigate the expression of 06- methylguanine- DNA- methyhransferase （MGMT） in human glioma cells and its relation with the patient＇ s survival time in order to judge the prognosis and guide the rational selection of postoperative chemotherapy. Methods 59 specimens of glioma were collected from the operative patients,and routinely fixed in 10% formalin,dehydrated conventionally and embedded in paraffin wax. Each specimen was cut to continuous sections of 5 um and stained with hematoxylin and eosin （H＆E） for confirming the histopathological type. The survival time of the patients was investigated. The expression of MGMT was detected by using EnVision immunohistochemical method. The semi- quantitative analysis was made on the results of the immunohistochemical SP staining according to Krajewska method. All the statistical analyses were made with SPSS17.0 for Windows software. X2 test was used in counting ranked data. Meanwhile, the survival time of patients and MGMT express were drawn a Kaplan- Meier survival curve and the log- rank test was performed. Results Heterogeneity existed in the expression of MGMT in human glioma. The positive rate of MGMT expression was different in different pathological grades,but the difference showed no statistical significance（P 〉 0.05）. The patients with positive MGMT expression had a shorter survival time than those with negative MGMT expression, showing statistical difference between them（P〈 0. 05）. Conclusion The expression of MGMT in human glioma has no relation with the pathological grades of glioma. MGMT expression may be used as an index for judging the prognosis.

关 键 词：胶质瘤 多药耐药 O6-甲基鸟嘌呤-DNA甲基转移酶 

分 类 号：R979.1[医药卫生—药品]