高效液相色谱法同时测定复方亚油酸乙酯胶丸中维生素C和烟酸含量  

Determination of Vitamin C and Nicotinic Acid in Compound Trivitamin and Linolic Acid Soft Capsules by HPLC

作　　者：沈黎 刘玉春 

机构地区：[1]安徽省阜阳市食品药品检验所,安徽阜阳236015

出　　处：《中国药业》2012年第14期50-51,共2页China Pharmaceuticals

摘　　要：目的建立测定复方亚油酸乙酯胶丸中维生素C和烟酸含量的高效液相色谱法。方法色谱柱为Waters Fire C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.004 mol/L庚烷磺酸钠溶液-冰醋酸(10∶90∶0.05),流速为1.0 mL/min,柱温为30℃,检测波长254 nm。结果维生素C质量浓度在12.09～241.8μg/mL范围内与峰面积线性关系良好(r=0.999 8,n=6),平均加样回收率为98.89%,RSD为1.09%(n=9);烟酸质量浓度在12.57～251.4μg/mL范围内与峰面积线性关系良好(r=1.000 0,n=6),平均加样回收率为98.17%,RSD为1.06%(n=9)。结论该方法简便快速,结果准确,可作为该产品质量控制方法。Objective To develop a method for the determination of vitamin C and nicotinic acid in compound trivitamin and linolic acid soft capsules. Methods The HPLC analysis was performed on Waters Fire C18 column（250 mm ×4. 6 mm,5 um） with mobile phase of methanol-0. 004 mol/L sodium heptanesulfonate- glacial acetic acid（10：90：0.05） ,the flow rate was 1.0 mL/min,the detection wavelength was at 254 nm and the column temperature was 30 ℃. Results The linear range of vitamin C was 12.09-241.8 ug/mL（ r = 0. 999 8, n = 6） 98.89% with RSD of 1.09% （ n = 9）. The linear range of nicotinic acid was 12.57 - 251.4 ug/mL（ r = 1. 000 0, n = 6） and the average recovery was 98.17% with RSD of 1.06% （n =9）. Conclusion This method is convenient, precise,reliable and suitable for the quality control of this preparation

关 键 词：复方亚油酸乙酯胶丸 维生素C 烟酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R972.6