月季品种杂3愈伤组织诱导条件的初步研究  

Study on Callus Induction Conditions of Rosa chinensis Variety Za3

作　　者：尤扬[1] 金典生[2] 

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003 [2]河南机电高等专科学校,河南新乡453000

出　　处：《湖北农业科学》2012年第13期2855-2857,共3页Hubei Agricultural Sciences

基　　金：河南科技学院重点科研基金资助项目(050122)

摘　　要：以月季(Rosa chinensis)品种杂3为试材,研究了培养基中激素浓度、光照条件及外植体取材时期和部位对愈伤组织诱导的影响。结果表明,MS培养基中添加2.5 mg/L 2,4-D和暗培养有利于月季愈伤组织的诱导;以小叶为外植体时愈伤组织诱导率高于以小复叶为外植体,且生长势较好。无菌苗生长2个月左右时从中部(顶生第6、7片叶)取样,生长3个月左右时从上部(顶生第3～5片叶)取样,小叶的愈伤组织诱导情况好于从其他部位取样。The Rosa chinensis variety Za3 was used to study the effects of hormone concentration in culture medium, illumi- nation condition, collecting period and position of explant on callus induction. Results showed that MS＋2.5 mg/L 2,4-D and dark culture was good for callus induction. Higher callus induction rate and better growth potential could be achieved if using leaflet other than trifoliolate leaves as explants. Leaflet collected from the central section （the sixth or seventh leaf from the peak） on two-month aseptic seedling or upper section （the third to the fifth leaf from the peak） on three-month aseptic seedling was better explant than the other parts.

关 键 词：月季(Rosa chinensis) 愈伤组织 诱导条件 

分 类 号：S685.12[农业科学—观赏园艺] S339.41[农业科学—园艺学]