血清白细胞介素-17、白三烯B4及IgE水平在哮喘患儿中的表达及意义  

The expression and clinical significance of the serum IL-17/LTB4/IgE in children with asthma

作　　者：马春艳[1] 王鑫[1] 宁立华[1] 张亚京[1] 陈华[1] 周芳[1] 郝锦丽[1] 周蕊[1] 

机构地区：[1]内蒙古医科大学第三附属医院儿科,包头014010

出　　处：《国际儿科学杂志》2012年第4期428-431,共4页International Journal of Pediatrics

摘　　要：目的探讨血清白细胞介素-17（IL-17）、白三烯B4（LTB4）及总IgE水平在支气管哮喘发病中的作用及其关系。方法研究组选择哮喘急性发作患儿60例（其中轻度组29例，中重度组31例），对照组选择健康儿童24例。应用酶联免疫吸附法检测各组儿童血清IL-17、LTB4水平，荧光酶联免疫法检测血清总IgE水平，哮喘患儿进行肺功能检测。全自动血液分析仪进行外周血中性粒细胞计数。结果（1）哮喘轻度组、中重度组与对照组血清IL-17水平分别为（1．15±0．10）μg／L、（2．80±2．30）μg/L、（0．83＋0．10）μg/L；LTB4水平分别为（2．22±1．01）μg／L、（8．79±9．36）μg/L、（1．94±1．13）μg/L；IgE水平分别为（123．70±86．94）μg／L、（322．27±332．28）μg/L、（24．27±7．64）μg／L，组间比较差异均有统计学意义（P〈0．001）；（2）哮喘轻度组、中重度组与对照组血清中性粒细胞百分比分别为（55．06±11．15）％、（64．44±11．87）％、（47．96±13．52）％，淋巴细胞百分比分别为（42．20±11．04）％、（33．93±10．02）％、（49．65±13．02）％，组间比较差异有统计学意义（P〈0．05）；（3）哮喘患儿血清IL-17与IgE水平呈正相关（P=0．004），哮喘患儿血清LTB4与IgE水平呈正相关（P=0．011），哮喘患儿血清IL-17与LTB4水平呈正相关（P=0．000）；（4）哮喘息儿血清IL-17、LTB4水平与FEV1（P=0．000）及PEF（P=0．000）均呈负相关，与中性粒细胞百分比呈正相关（P=0．000）。结论IL-17、LTB4及IgE在哮喘的急性发作及加重中起着重要作用，在哮喘发病中彼此相互影响，共同参与哮喘的病理生理改变。Objective To explore the impact of levels of serum IL-17, Leukotriene B4 and lgE on pathogenesis of childhood asthma. Methods Totally 60 children with asthma acute exacerbation （29 children with mild asthma,31 children with moderate-severe asthma） were selected as study group,24 healthy children were selected as control group. Serum IL-17 and LTB4 were measured with euryzemLinked immunosorbent assay,serum IgE was determined with enzyme-linked fluoroimmuneassay by pharmacia CAP Sytem,PMN was determined with automatic blood analyser,pulmonary function was measured in the study group. Results （ 1 ） The level of serum IL- 17 （ 1.15 ±0. 10 μg/L, 2. 80 ±2. 30 μg/L, 0. 83 ±0. 10 μg/L）, LTB4 （ 2. 22 ± 1.01 μg/L, 8. 79 ±9. 36 μg/L, 1.94 ±1.13 μg/L） and IgE（123.70±86. 94 μg/L,322. 27 ±332. 28 μg/L,24. 27 ±7.64 μg/L） were significantly different among mild asthma group, moderate-severe asthma group and control group（ P 〈 0. 001 ）. （ 2 ） The N% of mild asthma group, moderate-severe asthma group and study group were（55.06 ±11.15 ）%, （64.44 ±11.87） % , （47. 96 ±13.52）% , were（42. 20 ±11.04） % , （33.93 ±10. 02）% , （49. 65 ±13.02）% ,and there were significant differences in N% and L% between study group and control group（ P 〈 0. 05 ）. （3） There were significant positive correlations between the serum IL-17 levels and lgE ,LTB4 and lgE ,IL-17 and LTB4 in asthmatic children （P 〈 0. 05 ）. （4） There were significant negative correlcations between the level of serum IL- 17, LTB4 and FEV1, PEF （ P 〈 0. 001 ）. There were significant positive correlations between serum IL-17, LTB4 and N% （ P 〈0. 001 ）. （5） There were not correlations between the level of serum IgE and FEV1 ,PEF and N% in asthmatic children（ P 〉 0. 05 ）. Conclusion The levels of serum IL-17, LTB4 and IgE participated in pathogenesis on asthmatic children patients.

关 键 词：哮喘 白细胞介素17 白三烯B4 IGE 中性粒细胞 儿童 

分 类 号：R725.6[医药卫生—儿科]