低剂量西维因和氰戊菊酯农药联合染毒对大鼠子宫及机体氧化应激的影响  

Effects of Carbaryl and Fenvalerate in Combined on Uterus and Oxidative Stress in Rats

作　　者：李玲[1] 王秀琴[1] 董桂清[1] 李美莉[1] 马文涛[1] 

机构地区：[1]宁夏医科大学公共卫生学院劳动卫生与环境卫生学系宁夏生殖与遗传重点实验室省部共建生育力保持教育部实验室,宁夏银川750004

出　　处：《环境与健康杂志》2012年第7期609-611,F0003,共4页Journal of Environment and Health

基　　金：宁夏自然科学基金资助项目(NZ1099)

摘　　要：目的探讨低剂量西维因和氰戊菊酯联合染毒对雌性大鼠子宫系统和机体氧化应激的影响。方法将40只清洁级成年雌性SD大鼠按2×2析因设计随机分为4组,分别为阴性对照(玉米油)组、西维因单独染毒组(1/50LD50,11.2 mg/kg)、氰戊菊酯单独染毒组(1/50 LD50,9.02 mg/kg)和西维因(11.2 mg/kg)+氰戊菊酯(9.02 mg/kg)联合染毒组,每组10只。采用经口灌胃方式进行染毒,每天1次,每周6 d,连续染毒8周。末次染毒24 h后,测量大鼠体重和子宫重量,并计算子宫系数;测定血清中超氧化物歧化酶(SOD)、谷胱甘肽S转移酶(GST)活力和还原性谷胱甘肽(GSH)、丙二醛(MDA)含量以及子宫一氧化氮合成酶(NOS)活力。结果西维因和氰戊菊酯联合染毒对雌性大鼠体重、血清中GST水平均无交互作用(P>0.05);对大鼠子宫系数、血清中MDA含量均存在拮抗作用(P<0.05);对血清中SOD活力、GSH含量及子宫NOS活力存在协同作用(P<0.05,P<0.01)。结论西维因和氰戊菊酯联合染毒对大鼠子宫系统存在联合毒性作用,可能是通过破坏氧化-抗氧化系统的平衡和影响子宫组织中NOS的活力等导致子宫功能的降低。Objective To study the combined toxic effects of carbaryl and fenvalerate on uterus in rats. Methods According to 2×2 factorial analysis, forty healthy and clean adult female SD rats were randomly divided into four groups, including a control group （given corn oil） and three experimental groups：carbaryl （1/50 LD50,11.2 mg/kg, dissolved in coin oil）, fenvalerate （1/50 LD50,9.02 mg/kg, dissolved in coin oil） and carbaryl＋fenvalerate（11.2 mg/kg＋9.02 mg/kg, dissolved in coin oil）, 10 rats in each group, the treatment was conducted through garage, once a day, six days a week, for eight consecutive weeks. Body weight gain,uterus organ weights were determined and the organ coefficients were counted. The activities of SOD,GSH, GST,the contents of MDA and NOS in serum were measured, and the organization morphology change in uterus was observed. Results There was no interaction between carbaryl and fenvalerate on female rat body weight gain, the level of GST （P〉 0.05）. There was antagonism between carbaryl and fenvalerate on the rat uterine viscera coefficient and the level of MDA in the blood （P〈0.05）. There was synergy between carbaryl and fenvalerate on the levels of SOD, GSH and the activity of NOS （P〈0.05,P〈0.01）. Conclusion Carbaryl combined with fenvalerate can cause obvious toxic effecls on the uterine tissue in rats,maybe lower the uterine function through the destruction of oxidation-antioxidant system balance and influence in the vitality of uterine tissue NOS.

关 键 词：西维因 氰戊菊酯 子宫 

分 类 号：R994.6[医药卫生—毒理学]