结核杆菌耐热抗原和磷酸类抗原对人外周血γδ T细胞活化和增殖的比较  

Comparison of Mycobacterium tuberculosis heat-resistant antigens and phosphoantigens on activation and proliferation of human peripheral γδ T cells

作　　者：丁艳[1] 盛玲玲[1] 邱晓静[1] 徐丹[1] 李柏青[1] 

机构地区：[1]蚌埠医学院,免疫学教研室,感染与免疫安徽省重点实验室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2012年第7期745-747,共3页Journal of Bengbu Medical College

基　　金：国家科技重大专项基金资助项目(2008ZX100032 011)

摘　　要：目的:比较结核杆菌耐热抗原(Mtb-HAg)和磷酸类抗原(HDMAPP)刺激正常人外周血γδT细胞活化和增殖的特点。方法:健康成人外周血单个核细胞(PBMC,1×106/ml),分别加Mtb-HAg(5μg/ml)和HDMAPP(2×10-9mol/ml)进行刺激,同时给予IL-2(50 u/ml)维持细胞增殖,另外设立只加IL-2的对照组。培养10 d后,采用抗CD3-FITC和抗TCRγδ-PE荧光抗体标记细胞,流式细胞术检测γδT细胞的比例。结果:正常人PBMC经Mtb-HAg刺激扩增后γδT细胞在扩增细胞中的比例明显低于HDMAPP组(P<0.01),均明显高于IL-2对照组(P<0.01)。各个体对2种抗原刺激扩增γδT细胞呈线性正相关关系(R2=0.855 1,P<0.01)。结论:Mtb-HAg和HDMAPP均可优势激活人γδT细胞,且后者较前者有更强的活性。Objective：To compare the characteristics of Mycobacterium tuberculosis heat-resistant antigens （Mtb-HAg） and a kind of phosphoantigens,and HDMAPP on activation and proliferation of human peripheral blood γδ T cells. Methods：The peripheral blood mononuclear cells（PBMC） （1×10^6/ml）from healthy adults were cultured with Mtb-HAg （5 μg/ml） of HDMAPP （2×10^9 mol/ ml） ,and IL-2（50 u/ml） added to maintain cell proliferation. The group in which only IL-2 added was as control. After cultured for 10 days, γδ T cells were labelled with anti-human-CD3-FITC and anti-human-TCR-fS-PE, and detected by flow cytometry. Results：Mter amplification, the proportion of γδ T cells in Mtb-HAg activated group was significantly lower than that in HDMAPP group（ P 〈 0.05 ）. The proportion of γδ T ceils in these two groups were significantly higher than that in IL-2 control group（ P 〈0. 01 ）. Each individual had a linear correlation with the two kinds of antigen stimulated amplification of γδ T cells （ R2 = 0. 855 1, P 〈0. 01 ）. Conclusions ： Both Mtb-HAg and HDMAPP can preferentially activate human peripheral γδ T cells, and HDMAPP has a stronger activity.

关 键 词：结核杆菌耐热抗原 磷酸类抗原 ΓΔT细胞 

分 类 号：R378.911[医药卫生—病原生物学]