枸杞中维生素B_2固体粉末的荧光光谱检测研究  

Determination of the contents of VB_2 in Lycium barbarum L.powder with fluorescence spectrum method

作　　者：杨苗[1,2] 张伟刚[1] 杨萍果[3] 严肃源[1] 

机构地区：[1]南开大学现代光学研究所、光学信息技术科学教育部重点实验室,天津300071 [2]山西农业大学文理学院,山西太谷030801 [3]山西师范大学生命科学院,山西临汾041000

出　　处：《光电子．激光》2012年第8期1625-1630,共6页Journal of Optoelectronics·Laser

基　　金：国家自然科学基金(10974100,10674075,60577018);天津市应用基础与前沿技术研究计划重点项目(10JCZDJC24300);光学信息技术教育部重点实验室开放基金资助项目

摘　　要：利用日本岛津RF-5301PC荧光分光光度计,对枸杞子粉末中的维生素B2(VB2)直接进行荧光光谱检测,采集的光谱范围在530～650nm波段。采用MATLAB数值微分和高斯函数拟合方法,得到在激发波长为480nm、峰值波长为563nm时的荧光光谱信息;通过数值积分和线性拟合,得到荧光强度沿波长的积分与VB2含量呈良好的线性关系,其相关系数为0.998 43。实验测定枸杞粉末VB2含量为1.28±0.17μg/g,并阐释了枸杞子粉末荧光光谱红移的原因。研究表明:这种固体粉末荧光光谱检测方法具有快速、直接、简单和环保等优点,对药品成份检测、食品安全监测等领域的应用具有重要的参考价值。By using RF-5301PC series spectrofluorophotometer,the fluorescence spectrum of Lycium bar- barum L. powder is directly acquired to measure the content of vitamin De （VDe）. The collected its fluorescence spectrum is in the band of 530-650 nm. Through MATLAB-based numerical differential and Gaussian fitting algorithms, the fluorescent spectrum information with 563 nm peak under 480 nm excitation has been obtained,and after numerical integral calculation and linear fitting,it is found that there exists a linear relationship between the integral of fluorescent intensity by wavelength and the content of （R=0. 999843）. The content of VDe in Lycium barbarum L. powder is measured to he 1.28±0.17μg/g. The cause of red shift of the fluorescence spectrum of the Lycium barbarum I.. powder is illustrated. Our proposed VDe detection method has many advantages, such as high speed, directness, simplicity, and environment protection, and it is also a powerful tool that could be applied in food seeuritv detection.

关 键 词：荧光光谱 固体检测 枸杞粉末 维生素B2(VB2) 

分 类 号：O657.3[理学—分析化学]