格氏线虫表皮蛋白和分泌蛋白抑制昆虫免疫活性的比较  

Comparison of the Immunosuppressive Activity between Cuticle Coat Proteins and Secrete Proteins from Steinernema glaseri

作　　者：刘劲[1,2] 刘华[2] 袁京京[2] 刘峥[2] 杨秀芬[2] 张利莉[1] 邱德文[2] 曾洪梅[2] 

机构地区：[1]塔里木大学生命科学学院/新疆建设兵团塔里木盆地生物资源保护与利用重点实验室,阿拉尔843300 [2]中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室,北京100081

出　　处：《中国生物防治学报》2012年第3期400-407,共8页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31071741);948项目(2011-G4)

摘　　要：格氏线虫表皮蛋白和分泌蛋白都具有抑制昆虫免疫反应的能力,能够帮助侵染期线虫侵染寄主,但两者所含蛋白组分并不相同,从而导致蛋白活性也有所差异。本研究采用乙醇萃取和昆虫匀浆诱导,分别从侵染期的格氏线虫体表及分泌物中得到了表皮蛋白和分泌蛋白,并比较了这两者之间的异同。结果表明,无论是在昆虫体内还是体外,侵染期线虫表皮蛋白和分泌蛋白都具有抑制昆虫免疫反应的生物活性,并且分泌蛋白的活性更强。本研究为进一步研究线虫侵染与昆虫免疫间的相互关系和线虫抑制昆虫免疫反应的作用机理提供了科学佐证。Both surface coat proteins （SCPs） and secrete proteins （SPs） from Steinernema glaseri infective juveniles are able to suppress insect immune activities and were proved to play important roles in promoting infection of entomopathogenic nematodes. However, they are different in activities because of their different constituents. In this study, the SCPs were extracted with ethanol and the SPs were induced by Galleria me[lonella homogenates, then the similarity and difference between them were compared. Result indicated that no matter in vivo or in vitro, both kinds of proteins have the ability to suppress insect immunity, but the activity of SPs is more powerful than that of SCPs. Our studies provide scientific evidence for further study of the relationship between entomopathogenic nematode and insect, and the immunosuppressive mechanism of Steinernema glaseri.

关 键 词：格氏线虫 表皮蛋白 分泌蛋白 血细胞裂解 抗吞噬作用 

分 类 号：S432.45[农业科学—植物病理学] Q966[农业科学—农业昆虫与害虫防治]