血清学实验室检查在梅毒临床诊断中的应用研究  

Application of serology laboratory in clinical diagnosis of Syphilis

作　　者：游媛[1] 陈岚[1] 肖鄂[1] 

机构地区：[1]华中科技大学同济学院附属协和医院西区检验科,湖北武汉430056

出　　处：《吉林医学》2012年第20期4282-4284,共3页Jilin Medical Journal

摘　　要：目的:比较分析甲苯胺红不加热血清反应素试验(TRUST)、梅毒胶体金法(SYP)、双抗原夹心酶联免疫法(ELISA)三种实验室检查方法的诊断意义。方法:临床入选226例志愿者,通过梅毒螺旋体颗粒凝集试验(TPPA)检查,分为TP-PA(+)组(n=129)和TPPA(-)组(n=97),均采用TRUST、SYP和ELISAA检测其梅毒螺旋体抗体。结果:TPPA(+)组和TPPA(-)组之间的TRUST阳性率、SYP阳性率及ELISA阳性率均具有统计学差异(P<0.05);在TPPA(+)组中,与TRUST和SYP相比较,ELISA阳性率最高,高达96.12%(P<0.05)。而在TPPA(-)组中,与TRUST和ELISA相比较,SYP阳性率最高,为20.62%(P<0.05)。与TRUST和SYP相比较,ELISA的灵敏度和Youden指数最高(P<0.05)。与TRUST相比较,ELISA对不同阶段梅毒阳性率均显著升高(P<0.05)。结论:ELISA具有较高的敏感度和特异度、成本低、操作简易优点,可以替代TPPA作为梅毒血清学诊断的首选方法。Objective To investigate the diagnostic value of serology laboratory in the clinical diagnosis of syphilis. Method Total 226 cases were selected and divided into two groups,TPPA （ ＋ ）group （n = 129）, TPPA （ - ）group（ n = 97）. All used TRUST, SYP and ELISA to test their Treponema pallidum antibodies. Results There were significant differences in the positive rate of TRUST, SYP and ELISA be- tween the two groups （ P 〈 0. 05 ）. Compared with TRUST and SYP, the sensitivity and Youden index of ELISA were the highest （ P 〈 0. 05 ）. Meanwhile, compared with TRUST, the positive rate of different stages was higher in ELISA （P 〈 0. 05 ）. Conclusion This study indicates that ELISA has a high sensitivity and specificity,low cost, ease of operation advantages of alternative,it is the preferred method for diagnosis of Syphilis with replacing TPPA.

关 键 词：血清学实验室检查 梅毒 临床诊断 双抗原夹心酶联免疫法 

分 类 号：R446.6[医药卫生—诊断学]