银杏叶中总黄酮的提取  

Extraction of Total Flavones From Ginkgo Leaves

作　　者：刘德汞[1] 缑星[1] 刘晶晶[1] 曹书勤[1] 

机构地区：[1]信阳师范学院化学化工学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2012年第3期352-354,420,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：河南省科技攻关项目(112102310579)

摘　　要：分别以不同比例的四氢呋喃-乙醇、乙醇-水为溶剂,采用水浴回流和浸泡法提取银杏叶中总黄酮,用分光光度法测定提取率.结果表明:采用水浴回流法时,以体积比为3∶1的四氢呋喃/乙醇为溶剂,银杏叶质量(g)与溶剂体积(mL)比为1∶13.3、水浴温度为50℃、时间为2 h,提取率可达14.7%;采用浸泡法时,以体积比为3∶1的乙醇/水为溶剂,银杏叶质量(g)与溶剂体积(mL)比为1∶10、浸泡时间为24 h,提取率最高达9.3%.Total flavone was extracted by using two methods including water bath backflow and soaking with different solvent such as tetrahydrofuran-ethanol and ethanol as solvent, respectively. The extraction percentage was measured by spectrophotometry. The results showed that the extraction percentage of total flavones for water bath baekflow reached 14.7% when the volume ratio of tetrabydrofuran to ethanol was 3： 1, the ratio of ginkgo leaves mass （g） to solvent volume （mL） was 1： 13, water bath temperature was 50 ℃ and extraction time were 2 hours, and the extraction percentage of 9.3% for soaking method could be achieved when a volume ratio of ethanol to water was 3： 1, the ratio of ginkgo leaves mass （g） to solvent volume （mL） was 1： 10, and the soaking time was 24 hours.

关 键 词：银杏叶 黄酮 提取 水浴回流法 浸泡法 

分 类 号：O636.9[理学—高分子化学]