密骨方对去势大鼠所致骨质疏松性骨折白细胞介素-1的影响  

Effect of density bone prescription on interleukin-1 in ovariectomized rats with osteoporosis-induced fracture

作　　者：石大玲[1] 汪学红[2,4] 姜婷[1] 何学斌[3] 沈霖[4] 

机构地区：[1]华中科技大学同济医学院,武汉430030 [2]武汉体育学院 [3]华中科技大学同济医学院附属梨园医院 [4]华中科技大学同济医学院附属协和医院

出　　处：《中华实验外科杂志》2012年第7期1254-1256,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察密骨方对骨质疏松性骨折（OPF）白细胞介素-1（IL-1）的影响。方法将90只雌性Wistar大鼠随机分为对照组（Sham），模型组（OVX）与密骨方治疗组（MGF），OVX组及MGF组行卵巢摘除术，同时MGF组和OVX组大鼠均致其右侧股骨上I／3处骨折。MGF组按100mg／（kg·d）的剂量灌胃给药，Sham组和OVX组给予同体积的生理盐水，每日1次，疗程分别为2、4、6周，采用免疫组织化学法观察骨折端IL—1的表达。结果OVX组IL-1在骨折端的表达随时间的延长（2、4、6周）而显著升高（0．20660±0．03195、0．20790±0．03347、0．21120±0．02429，P〈0．05），而MGF组IL-1在骨折端的表达随时间的延长而显著降低（0．21580±0．02800、0．20870±0．03252、0．20480±0．03465，P〈0．05）；同时MGF组第6周IL-1在骨折端的表达显著低于OVX组（P〈0．01）。结论MGF能显著降低大鼠OPF端IL-1的合成分泌，从而抑制破骨细胞的活性，促进骨折愈合。Objective To study the effects of density bone prescription, on interleukin （IL）-I in ovariectomized rats with osteoporosis-induced fracture （OPF）. Methods Ninety female Wistar rats were randomly allocated into normal control group （sham）, mode/group （OVX） and MGF group. The rats in OVX group and MGF group were ovariectomized, meanwhile their right femur fractures at the position of 1/ 3 were made. The rats in MGF group were given MGF by intragastric gavage at the dose of 100 mg/kg, and the rest two groups were given normal saline at the same dose, once a day, for 2, 4 or 6 weeks. The ex- pression of IL-1 in the fracture ends was detected by using immunohistochemistry. Results With time passing, the expression of IL-1 at the fracture ends in OVX group was obviously increased （P 〈 0.05 ）, in MGF groups it was significantly decreased （ P 〈 0. 05-P 〈 0. 01 ）. The expression of IL-2 at the fracture ends in MGF group was significantly lower than in OVX group （ P 〈 0. 01 ）. Conclusion MGF could sig- nificantly reduce the IL-1 synthesis and secretion at the OPF ends, thereby inhibiting osteoclast activity, and promoting fracture healing.

关 键 词：骨质疏松性骨折 密骨方 白细胞介素-1 

分 类 号：R683[医药卫生—骨科学]