大鼠放射性皮肤溃疡组织中p53,MDM2蛋白高表达病理研究(英文)  

OVEREXPRESSION OF p53, MDM2 PROTEINS IN SOME ATR RADIATION-IMDUCED SKIN ULCERS

作　　者：谷庆阳[1] 高亚兵[1] 李永兰 杨志祥 周洁[4] 王德文[1] 崔玉芳[1] 赵坡[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]武警北京二总队医院,北京100039 [3]北京北太平路医院外科,北京100850 [4]军事医学科学院微生物流行病研究所,北京100850

出　　处：《辐射研究与辐射工艺学报》2000年第3期187-192,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金资助!(39470229)

摘　　要：研究大鼠放射性皮肤溃疡组织中ｐ５３；ＭＤＭ２表达。采用１４０只Ｗｉｓｔａｒ大鼠进行局部照射制备放射性皮肤溃疡动物模型，观察病变一年，然后采用ｐ５３（Ｇｏａｔ），ＭＤＭ２（兔）多克隆抗体和ＳＰ免疫组化方法检测皮肤溃疡组织中ｐ５３，ＭＤＭ２的表达情况。结果表明；ｐ５３， ＭＤＭ２的高表达阳性率分别为 ９．７％（７／７２）、 １９．４％（１４／７２），两者阳性部位主要见于增生肥大的鳞状上皮细胞核、间质成纤维细胞及血管内皮细胞核。ｐ５３，ＭＤＭ２基因的变化及蛋白的高表达可能与放射性皮肤溃疡癌变及不愈合有关。An animal model of radiation-induced skin ulcer was set up with 140 rats, which were locally irradiated with 35～55Gy γ-rays. The pathological changes were observed for 1 year. Immunohistochemical studies were performed in 72 rat radiation skin ulcer specimens using anti-p53 and anti-MDM2 protein polyclonal antibodies. The results showed that the positive rate for overexpression of p53 protein was 9.7%, and for that of MDM2 was 19.4%. The overexpression of p53 was mainly seen in the nuclei of activated squamous epithelial cells, and in fibroblasts, endotheliocytes in deeper part of the skin ulcers. The overexpression of MDM2 had the same localizations. It's suggested that the changes of p53 and MDM2, genes and proteins, may be related to the cancer transformation and poor healing of radiation-induced skin ulcers.

关 键 词：大鼠 放射性皮肤溃疡 P53 MDM2 蛋白 基因表达 

分 类 号：R818.895[医药卫生—放射医学]