小鼠骨髓细胞及骨髓基质细胞混合输注对受致死剂量照射小鼠造血重建的影响  

THE EFFECT OF MIXED INFUSION OF BONE MARROW CELLS AND BONE MARROW STROMAL CELLS ON HEMATOPOIETIC RECONSTITUTION IN LETHALLY IRRADIATED MICE

作　　者：刘静[1] 纪树荃[1] 秦凤华[2] 陈惠仁[1] 柳小兰 耿玉 

机构地区：[1]空军总医院血液科,北京100036 [2]北京放射医学研究所,北京1000850

出　　处：《辐射研究与辐射工艺学报》2000年第3期218-222,共5页Journal of Radiation Research and Radiation Processing

摘　　要：为观察小鼠骨髓细胞（ＢＭＣ）及骨髓基质细胞（ＢＭＳＣ）混合输注对受致死剂量照射 小鼠造血重建的影响，给予受致死剂量照射的 Ｂａｌｂ／ｃ 小鼠输注同基因 ＢＭＣ１×１０７个及经体 外扩增的同基因原代 ＢＭＳＣ ２×１０５个，并与单纯骨髓移植组进行了比较。结果表明，移植后第 １４天外周血白细胞、血小板回升较快，第 １５天、第 ２０天粒－单系祖细胞（ＣＦＵ－ＧＭ）、红系 祖细胞（ＢＦＵ－Ｅ、 ＣＦＵ－Ｅ）、造血干细胞（ＣＦＵ－Ｓ）明显高于后者，第 ２０天已达正常。说明原 代ＢＭＳＣ不仅可以移植，而且能够加快造血重建；提高移植效果。To observe the effect of mixed infusion of bone marrow and bone marrow stromal cells on hematopoietic reconstitution in lethally irradiated mice, Balb/c mice irradiated lethally received 1×107 syngeneic bone marrow cells and 2×105 syngeneic bone marrow stromal cells via the intravenous route. As compared with the simple DMT group, the WBC and the BPC in peripheral blood in mixed infusion group recover more quickly on day 14 after BMT and BMSCT. The numbers of CFU-GM, BFU-E, CFU-E, CFU-S in mixed infusion group are higher than that of the simple BMT group on day 15 and day 20 after BMT and BMSCT. Conclusion: primary cultured bone marrow stromal cells not only is transplantable, but also can accelerate hematopoietic reconstitution.

关 键 词：骨髓移植 骨髓基质细胞 体外圹增 造血重建 

分 类 号：R457.7[医药卫生—治疗学]