鸡胚发育中神经钙黏蛋白对头部神经嵴细胞分层的作用(英文)  

Role of N-cadherin in cranial neural crest delamination during chick embryogenesis

作　　者：文皓旻[1] 李艳[1,2] 王广[1,2] 张笑坛[1,2] 王晓钰[1,2] 张兆龙[1,2] 杨雪松[1,2] 

机构地区：[1]暨南大学再生医学教育部重点实验室,生命科学技术学院,广东广州510632 [2]暨南大学医学院,广东广州510632

出　　处：《中国病理生理杂志》2012年第7期1269-1274,共6页Chinese Journal of Pathophysiology

基　　金：Supported by NSFC(No.30971493;.31071054);“973 Project”(No.2010CB529702);the Fundamental Research Funds for the Central Universities(No.21610601)

摘　　要：目的:阐明神经钙黏蛋白(N-cadherin)在神经嵴细胞分层中的作用。方法:利用原位杂交鉴定N-cadherin在神经管上的表达。转染过表达N-cadherin的野生型N-cadherin(wild-type N-cadherin,wt-N-cad)或阻断N-cadherin表达的结构域缺失型N-cadherin(dominant-negative N-cadherin,dn-N-cad),利用免疫组化方法检测头部神经嵴细胞的迁移变化。结果:无论是过表达N-cadherin还是下调N-cadherin表达,头部神经嵴细胞迁移都受到了影响。结论:头部神经嵴细胞的分层和迁移依赖于神经管上N-cadherin的表达。AIM： To investigate the role of N - cadberin in the delamination of neural crest cells. METHODS： The normal expression of N - eadherin in neural tube was identified using in situ hybridization. The cells with N - eadherin over expression were obtained by transfection of wild -type N -cadherin （ wt - N -cadherin） , and the cells with N -cadber- in silencing expression were obtained by transfeetion of dominant - negative N - cadherin （ dn - N - eadherin）. The migra- tion of cranial neural crest cells was determined by the technique of immunohistochemistry. RESULTS： Either overexpres- sion or down - regulation of N - eadherin significantly affected the migration of cranial neural crest cells. CONCLUSION： Delamination and migration of the cranial neural crest cells rely on the relative N - eadherin expression in the neural tube during ncurulation.

关 键 词：神经嵴 神经钙黏蛋白 迁移 分层 鸡胚 

分 类 号：R285.5[医药卫生—中药学]