终末糖基化产物对神经小胶质细胞IL-1β、IL-6表达的影响  

Effects on Expression of IL-1β,IL-6 in Cultured Bv-2 Cell Stimulated by Advanced Glycation end Products

作　　者：张媛元 杜晓红[2] 毛瑞阳[2] 刘毅[3] 

机构地区：[1]浙江省温州市平阳县第二人民医院肾内科,平阳325000 [2]温州医学院第一附属医院干部科,温州325000 [3]温州医学院第一附属医院肾内科,温州325000

出　　处：《中国中西医结合肾病杂志》2012年第7期587-590,I0004,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：温州市科技局基金资助项目(No.Y20070010)

摘　　要：目的:研究终末糖基化产物(AGEs)对神经小胶质细胞白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)表达的影响。方法:以不同浓度、不同时间AGEs刺激体外培养的小鼠小胶质瘤细胞(BV-2细胞),观察细胞形态的变化,应用逆转录聚合酶链反应(RT-PCR)检测细胞内IL-1β、IL-6mRNA水平,应用酶联免疫吸附法(ELISA)测定细胞培养上清液中IL-1β、IL-6的蛋白含量。结果:与空白组和AGEs对照物组比较,AGEs组细胞活化呈阿米巴样,胞核大而圆,核仁明显,胞浆内颗粒物增多,以300mg/L作用18h组效果最显著。与空白组和AGEs对照物组比较,作用时间相同时,AGEs浓度为50mg/L时即可见细胞的IL-1β、IL-6mRNA水平明显增加(P<0.05或P<0.01),浓度为150mg/L时,细胞培养上清液中IL-1β、IL-6的蛋白含量明显增加(P<0.05);作用浓度相同时,2h即可见细胞的IL-1βmRNA水平明显增加(P<0.05),6h见细胞的IL-6mRNA水平和细胞培养上清液中IL-1β、IL-6的蛋白含量明显增加(P<0.05或P<0.01),其中以300mg/L作用18h组效果最显著(P<0.05)。结论:AGEs刺激可使小胶质细胞内IL-1β、IL-6高表达。Objective：To investigate the effect of the expression of IL-1β and IL-6 in BV-2 cell stimulated by different concentration of advanced glycation end products for different time.Methods：The cultured BV-2 cells were treated with different concentrations of advanced glycation end products（50,150,300,600,800 mg/L） for different time.Observe the morphologic change of the cells by inverted microscope,and evaluate the concentration of IL-1βand IL-6 in the cell culture supernate by enzyme linked immunosorbent assay（ELISA）.Well,mRNA levels of the inflammatory cytokines interleukin IL-1βand IL-6 were analysed by reverse transcriptasepolymerase chain reaction（RT-PCR）.Results：Compared with the control group,after 18 h treatment,in the group of advanced glycation end products（300 mg/L）,the cells is easy to assemble,cell body and nucleus become bigger,neurodendron is obvious,and the particulate matter in the intracytoplasm increased.Furthermore,The levels of IL-1β and IL-6 protein in supernatants were increased gradually with the increase of advanced glycation end products concentration or action time,get the most notable effect at 18h and 300mg/L inn a time and dose-dependent manner.These results were observed with semi-quantitative RT-PCR of IL-1β and IL-6 mRNA.Conclusion：Advanced glycation end products can stimulate the expression of IL-1β and IL-6 at the levels of protein and mRNA in gitter cells.

关 键 词：终末糖基化产物 白细胞介素-1Β 白细胞介素-6 神经小胶质细胞 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]