甜菜线粒体atp6基因转录本的RNA编辑位点研究  

The Study of RNA Editing Sites of the atp6 Gene Transcripts in Sugar-beet Mitochondria

作　　者：施真[1] 程大友[1,2] 罗成飞[2] 刘巧红[2] 史淑芝[2] 崔杰[2] 

机构地区：[1]哈尔滨工业大学生命科学与技术学院,哈尔滨150001 [2]哈尔滨工业大学食品科学与工程学院,哈尔滨150001

出　　处：《分子植物育种》2012年第4期428-432,共5页Molecular Plant Breeding

基　　金：国家自然科学基金(30771373);黑龙江省自然科学基金(C200807)共同资助

摘　　要：RNA编辑现象普遍存在于高等植物的线粒体中,是线粒体中产生功能蛋白所不可或缺的加工步骤。以甜菜细胞质雄性不育系DY5-CMS及其保持系DY5-O为材料,通过PCR、RT-PCR以及PCR产物直接测序等方法,对甜菜线粒体atp6基因转录本的RNA编辑位点进行了研究,以期探寻其与细胞质雄性不育之间的关系。结果表明,atp6基因转录本的保守区域共有8个编辑位点,均发生在密码子的第一、二位点上,这些位点的编辑将导致氨基酸种类的变化,如第二个编辑位点上发生的由丝氨酸(疏水参数0.8)到亮氨酸(疏水参数3.8)的转变。通过分析可看出atp6基因RNA编辑有着改变蛋白质疏水性的作用,同时它能增加物种间的保守性。The RNA editing event exits extensively in the higher plant mitochondrial and is an indispensable process for prgducing functional proteins in mitochondria. Using the sugar-beet Cytoplasmic Male Sterility line DYS-CMS and the maintainer line DY5-O as the research materials, the editing sites of the atp6 gene transcripts in the sugar-beet mitochondrial was studied by PCR, RT-PCR and PCR products direct sequencing methods to explore its relationship with cytoplasmic male sterility. The results showed that there were eight editing sites in the conservative region of atp6 gene transcripts and all of the editing sites were located at the first or the second position of codons, which resulted in the change of the amino acid type, such as the transformation of Ser to Leu in the second editing site. Through this analysis, the RNA editing of atp6 gene was shown to change the protein＇s hydrophobicity and increased the conservation among species at the same time.

关 键 词：甜菜 线粒体 RNA编辑 atp6基因 细胞质雄性不育 

分 类 号：S566.3[农业科学—作物学]